Mechanosensitive Endocytosis of High-Stiffness, Submicron Microgels in Macrophage and Hepatocarcinoma Cell Lines
| Autor: | Alexei V. Tivanski, Kendra J. Bell, Terra M. Kruger, Brittany E. Givens, Aliasger K. Salem, Lewis L. Stevens, Thiranjeewa I. Lansakara, Himansu Mohapatra |
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| Rok vydání: | 2022 |
| Předmět: |
Range (particle radiation)
Materials science Biochemistry (medical) Biomedical Engineering 02 engineering and technology General Chemistry Nanoindentation 010402 general chemistry 021001 nanoscience & nanotechnology Endocytosis 01 natural sciences 0104 chemical sciences Styrene Biomaterials chemistry.chemical_compound chemistry Chemical engineering Particle Mechanosensitive channels Particle size Surface charge 0210 nano-technology |
| Zdroj: | ACS applied bio materials. 1(5) |
| ISSN: | 2576-6422 |
| Popis: | The mechanical properties of submicron particles offer a unique design space for advanced drug-delivery particle engineering. However, the recognition of this potential is limited by a poor consensus about both the specificity and sensitivity of mechanosensitive endocytosis over a broad particle stiffness range. In this report, our model series of polystyrene-co-poly(N-isopropylacrylamide) (pS-co-NIPAM) microgels have been prepared with a nominally constant monomer composition (50 mol % styrene and 50 mol % NIPAM) with varied bis-acrylamide cross-linking densities to introduce a tuned spectrum of particle mechanics without significant variation in particle size and surface charge. While previous mechanosensitive studies use particles with moduli ranging from 15 kPa to 20 MPa, the pS-co-NIPAM particles have Young’s moduli (E) ranging from 300 to 700 MPa, which is drastically stiffer than these previous studies as well as pure pNIPAM. Despite this elevated stiffness, particle uptake in RAW264.7 murine macro... |
| Databáze: | OpenAIRE |
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