Tyrosinase Depletion Prevents the Maturation of Melanosomes in the Mouse Hair Follicle
| Autor: | Victoria Eby, Kazumasa Wakamatsu, Thomas J. Fielder, Anand K. Ganesan, Daniel L. Gillen, Grant R. MacGregor, Shosuke Ito, Raymond E. Boissy, Elyse K. Paterson |
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| Přispěvatelé: | Hou, Ling |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty General Science & Technology Tyrosinase lcsh:Medicine Human skin Skin Pigmentation Biology Inbred C57BL Cell Line Melanin 030207 dermatology & venereal diseases 03 medical and health sciences Pigment Mice 0302 clinical medicine Cell Line Tumor Gene expression medicine Animals lcsh:Science 030304 developmental biology Melanosome Skin Melanins 0303 health sciences Multidisciplinary Tumor Melanosomes integumentary system Monophenol Monooxygenase lcsh:R Hair follicle Cell biology Mice Inbred C57BL medicine.anatomical_structure Cell culture visual_art visual_art.visual_art_medium lcsh:Q sense organs Hair Follicle Research Article |
| Zdroj: | Paterson, EK; Fielder, TJ; MacGregor, GR; Ito, S; Wakamatsu, K; Gillen, DL; et al.(2015). Tyrosinase Depletion Prevents the Maturation of Melanosomes in the Mouse Hair Follicle. PLOS ONE, 10(11). doi: 10.1371/journal.pone.0143702. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/3k80t4w2 PLoS ONE, Vol 10, Iss 11, p e0143702 (2015) PloS one, vol 10, iss 11 PLoS ONE |
| DOI: | 10.1371/journal.pone.0143702. |
| Popis: | The mechanisms that lead to variation in human skin and hair color are not fully understood. To better understand the molecular control of skin and hair color variation, we modulated the expression of Tyrosinase (Tyr), which controls the rate-limiting step of melanogenesis, by expressing a single-copy, tetracycline-inducible shRNA against Tyr in mice. Moderate depletion of TYR was sufficient to alter the appearance of the mouse coat in black, agouti, and yellow coat color backgrounds, even though TYR depletion did not significantly inhibit accumulation of melanin within the mouse hair. Ultra-structural studies revealed that the reduction of Tyr inhibited the accumulation of terminal melanosomes, and inhibited the expression of genes that regulate melanogenesis. These results indicate that color in skin and hair is determined not only by the total amount of melanin within the hair, but also by the relative accumulation of mature melanosomes. |
| Databáze: | OpenAIRE |
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