Structural and Functional Characterization of the FGF Signaling Pathway in Regeneration of the Polychaete Worm Alitta virens (Annelida, Errantia)
| Autor: | Alexandra Y. Shalaeva, R. P. Kostyuchenko, Vitaly V. Kozin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
animal structures Receptor expression Annelida Cell Morphogenesis Nereis Biology QH426-470 Fibroblast growth factor SU5402 Article axis elongation 03 medical and health sciences 0302 clinical medicine evolution fibroblast growth factor medicine Genetics Animals Regeneration Axis elongation Genetics (clinical) annelids Cell Proliferation Cell growth blastema induction Regeneration (biology) dedifferentiation segmentation invertebrates Receptors Fibroblast Growth Factor Cell biology Fibroblast Growth Factors 030104 developmental biology medicine.anatomical_structure Ventral nerve cord 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Genes Genes, Vol 12, Iss 788, p 788 (2021) Volume 12 Issue 6 |
| ISSN: | 2073-4425 |
| Popis: | Epimorphic regeneration of lost body segments is a widespread phenomenon across annelids. However, the molecular inducers of the cell sources for this reparative morphogenesis have not been identified. In this study, we focused on the role of fibroblast growth factor (FGF) signaling in the posterior regeneration of Alitta virens. For the first time, we showed an early activation of FGF ligands and receptor expression in an annelid regenerating after amputation. The expression patterns indicate that the entire regenerative bud is competent to FGFs, whose activity precedes the initiation of cell proliferation. The critical requirement of FGF signaling, especially at early stages, is also supported by inhibitor treatments followed by proliferation assay, demonstrating that induction of blastemal cells depends on FGFs. Our results show that FGF signaling pathway is a key player in regenerative response, while the FGF-positive wound epithelium, ventral nerve cord and some mesodermal cells around the gut could be the inducing tissues. This mechanism resembles reparative regeneration of vertebrate appendages suggesting such a response to the injury may be ancestral for all bilaterians. |
| Databáze: | OpenAIRE |
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