Dinucleosome specificity and allosteric switch of the ISW1a ATP-dependent chromatin remodeler in transcription regulation
| Autor: | Blaine Bartholomew, Swetansu K. Hota, Jim Persinger, Lola Olufemi, Saurabh K. Bhardwaj, Solomon G. Hailu, Soumyadipta Kundu, Sandipan Brahma |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Xenopus Science Allosteric regulation General Physics and Astronomy Chromatin remodelling Saccharomyces cerevisiae General Biochemistry Genetics and Molecular Biology Article Histones 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Transcription (biology) Salmon Histone H2A Nucleosome Animals lcsh:Science skin and connective tissue diseases Histone Acetyltransferases Adenosine Triphosphatases Multidisciplinary biology Promoter General Chemistry Histone acetyltransferase Chromatin Assembly and Disassembly Cell biology Chromatin Nucleosomes DNA-Binding Proteins 030104 developmental biology chemistry Gene Expression Regulation Enzyme mechanisms biology.protein lcsh:Q sense organs 030217 neurology & neurosurgery DNA Transcription Factors |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Over the last 3 decades ATP-dependent chromatin remodelers have been thought to recognize chromatin at the level of single nucleosomes rather than higher-order organization of more than one nucleosome. We show the yeast ISW1a remodeler has such higher-order structural specificity, as manifested by large allosteric changes that activate the nucleosome remodeling and spacing activities of ISW1a when bound to dinucleosomes. Although the ATPase domain of Isw1 docks at the SHL2 position when ISW1a is bound to either mono- or di-nucleosomes, there are major differences in the interactions of the catalytic subunit Isw1 with the acidic pocket of nucleosomes and the accessory subunit Ioc3 with nucleosomal DNA. By mutational analysis and uncoupling of ISW1a’s dinucleosome specificity, we find that dinucleosome recognition is required by ISW1a for proper chromatin organization at promoters; as well as transcription regulation in combination with the histone acetyltransferase NuA4 and histone H2A.Z exchanger SWR1. Here the authors show that the preference of yeast chromatin remodeler ISW1a for dinucleosomes hinges on conformational changes that occur in the transition from binding mononucleosomes to dinucleosomes. These changes are critical for ISW1a organizing chromatin at promoters and regulating transcription in conjunction with other chromatin remodelers. |
| Databáze: | OpenAIRE |
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