The antitumor effect of a new docetaxel-loaded microbubble combined with low-frequency ultrasound in vitro: preparation and parameter analysis
| Autor: | Bing Wang, Hui Zhang, Li-Fang Tan, Hong Ai, Xin-Liang Shen, Yiping Li, Xing-Hua Zhao, Jing Jing, Yi-Ran Liao, Qiang Sun, Shu-Ting Ren, Xiao-Ning Kang |
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| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
animal structures Pharmaceutical Science Antineoplastic Agents Docetaxel Dogs Drug Delivery Systems Drug Stability Cell Line Tumor medicine Animals Humans Pharmacology (medical) Ultrasonics Target therapy Cell Proliferation Ultrasonography Pharmacology Microbubbles Chemistry business.industry Organic Chemistry Water Hydrogen-Ion Concentration Lipids In vitro Low frequency ultrasound Liver Solubility Parameter analysis Molecular Medicine Water chemistry Taxoids Radiology business Biotechnology Biomedical engineering medicine.drug |
| Zdroj: | Pharmaceutical research. 30(6) |
| ISSN: | 1573-904X |
| Popis: | To develop a novel docetaxel (DOC)-loaded lipid microbubbles (MBs) for achieving target therapy and overcoming the poor water-solubility drawback of DOC.A novel DOC-loaded microbubble (DOC + MB) was prepared by lyophilization and the physicochemical properties including ultrasound contrast imaging of the liver were measured. The anti-tumor effect of the DOC + MBs combined with low-frequency ultrasound (LFUS; 0.8 Hz, 2.56 W/cm², 50% cycle duty) on the DLD-1 cancer cell line was examined using an MTT assay.The physicochemical properties of the two tested formats of DOC + MBs (1.0 mg and 1.6 mg) was shown: concentration, (6.74 ± 0.02) × 10⁸ bubbles/mL and (8.27 ± 0.15) × 10⁸ bubbles/mL; mean size, 3.296 ± 0.004 μm and 3.387 ± 0.005 μm; pH value, 6.67 ± 0.11 and 6.56 ± 0.05; release rate, 3.41% and 12.50%; Zeta potential, -37.95 ± 7.84 mV and -44.35 ± 8.70 mV; and encapsulation efficiency, 54.9 ± 6.21% and 46.3 ± 5.69%, respectively. Compared with SonoVue, the DOC + MBs similarly enhanced the echo signal of the liver imaging. The anti-tumor effect of the DOC + MBs/LFUS group was significantly better than that of DOC alone and that of the normal MBs/LFUS groups.The self-made DOC + MBs have potential as a new ultrasound contrast agent and drug-loaded microbubble, and can obviously enhance the antitumor effect of DOC under LFUS exposure. |
| Databáze: | OpenAIRE |
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