A Long Cytoplasmic Loop Governs the Sensitivity of the Anti-viral Host Protein SERINC5 to HIV-1 Nef
Autor: | Heinrich G. Göttlinger, Weiwei Dai, Yuanfei Wu, Yoshiko Usami |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Cytoplasm viruses Human immunodeficiency virus (HIV) Antiviral protein restriction factor medicine.disease_cause General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences SERINC5 medicine Humans nef Gene Products Human Immunodeficiency Virus lcsh:QH301-705.5 Host protein Infectivity Nef 030102 biochemistry & molecular biology Chemistry Membrane Proteins virus diseases Cell biology 030104 developmental biology lcsh:Biology (General) Multipass transmembrane protein HIV-1 Intracellular |
Zdroj: | Cell Reports, Vol 22, Iss 4, Pp 869-875 (2018) |
ISSN: | 2211-1247 |
Popis: | We recently identified the multipass transmembrane protein SERINC5 as an antiviral protein that can potently inhibit HIV-1 infectivity and is counteracted by HIV-1 Nef. We now report that the anti-HIV-1 activity, but not the sensitivity to Nef, is conserved among vertebrate SERINC5 proteins. However, a Nef-resistant SERINC5 became Nef sensitive when its intracellular loop 4 (ICL4) was replaced by that of Nef-sensitive human SERINC5. Conversely, human SERINC5 became resistant to Nef when its ICL4 was replaced by that of a Nef-resistant SERINC5. In general, ICL4 regions from SERINCs that exhibited resistance to a given Nef conferred resistance to the same Nef when transferred to a sensitive SERINC, and vice versa. Our results establish that human SERINC5 can be modified to restrict HIV-1 infectivity even in the presence of Nef. |
Databáze: | OpenAIRE |
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