Astrocytes express Mxi2, a splice isoform of p38MAPK
| Autor: | Gonzalo Sánchez-Tejeda, Juan Pablo Albar, Sergio Ciordia, Enrique Vacas, María Amparo Albert, Luis A. López-Fernández, Silvia Juárez, Isabel Suárez, Benjamín Fernández, Guillermo Bodega |
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| Rok vydání: | 2009 |
| Předmět: |
Histology
Physiology Immunoprecipitation Blotting Western Biology Serine Mitogen-Activated Protein Kinase 14 Extracellular medicine Animals Protein Isoforms Rats Wistar Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Kinase Cell Biology General Medicine Molecular biology Cell biology Rats Blot Cytosol Alternative Splicing Protein Transport medicine.anatomical_structure Astrocytes Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Intracellular Astrocyte Subcellular Fractions |
| Zdroj: | Journal of molecular histology. 40(5-6) |
| ISSN: | 1567-2387 |
| Popis: | Mitogen-activated protein kinases (MAPKs) are a superfamily of cytoplasmic serine/threonine kinases that transduce many types of extracellular stimuli into cellular responses. p38MAPK is a member of this family with its active form in a diphosphorylated state (p38MAPKdiP). Two strong anti-p38MAPKdiP immunoreactive bands (apparent molecular weight 38 and 34 kDa) were detected by Western blotting in cultured astrocytes. Using a specific antibody and employing immunoprecipitation procedures and SELDI-TOF analysis, the 34 kDa band was found to correspond to Mxi2, a splice variant of p38MAPK; cultured astrocytes therefore express Mxi2. Separate protein extractions of different subcellular fractions, and fluorescent immunovisualisation employing confocal microscopy, showed Mxi2 to have a non-nuclear, cytosolic distribution in the studied cells. ERK1/2, protein whose intracellular distribution is influenced by Mxi2, showed the same cytoplasmic pattern than Mxi2. |
| Databáze: | OpenAIRE |
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