Synergistic bactericidal effect of nisin and phytic acid against Escherichia coli O157:H7
| Autor: | Ge Zhao, Paul Joseph Kempen, Tao Zheng, Tim Holm Jakobsen, Shuangqing Zhao, Liuyan Gu, Christian Solem, Peter Ruhdal Jensen |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Zhao, G, Kempen, P J, Zheng, T, Jakobsen, T H, Zhao, S, Gu, L, Solem, C & Ruhdal Jensen, P 2023, ' Synergistic bactericidal effect of nisin and phytic acid against Escherichia coli O157:H7 ', Food Control, vol. 144, 109324 . https://doi.org/10.1016/j.foodcont.2022.109324 Zhao, G, Kempen, P J, Zheng, T, Jakobsen, T H, Zhao, S, Gu, L, Solem, C & Ruhdal Jensen, P 2022, ' Synergistic bactericidal effect of nisin and phytic acid against Escherichia coli O157:H7 ', Food Control, vol. 144, 109324 . https://doi.org/10.1016/j.foodcont.2022.109324 |
| DOI: | 10.1016/j.foodcont.2022.109324 |
| Popis: | The food industry must prevent food contamination caused by pathogenic Escherichia coli strains as they pose a severe public health threat worldwide and lead to unimaginable economic losses. In this study, the synergistic antibacterial activity in vitro of nisin and phytic acid (PA), a natural metal chelate, against 5 foodborne isolates of E. coli were evaluated by a checkerboard assay. Checkerboard assay showed that nisin and PA showed a synergistic effect (FICI E. coli O157:H7 was investigated. Results showed the number of planktonic and biofilm cells inactivated in groups treated with a combination of 0.512 mg/mL nisin plus 1 × MIC or 2 × MIC PA was significantly higher than the sum of groups treated alone (P < 0.05). Electron scanning microscopy studies revealed that the nisin plus PA treatment had a severe effect on E. coli O157:H7, which after treatment cell morphology appeared deflated with large invaginations. The effect of nisin plus PA against E. coli O157:H7 was furthermore assessed on cold-stored beef, where a similar synergistic bactericidal effect was observed. The studies demonstrate the great potential of the food-approved nisin to control the growth of undesired E. coli pathogens in foods. We used PA to overcome the permeability barrier of the outer membrane to nisin, but other food-grade metal ion chelators such as polyphosphate most likely could be used as well. |
| Databáze: | OpenAIRE |
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