An improved method for the heterologous production of soluble human ribosomal proteins in Escherichia coli
| Autor: | Praveen G. Vadakkedath, Amy Lai, Danilo Correddu, Ivanhoe K. H. Leung, J. M. Lopez, Paris R. Watson, Jane I. Pernes |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Ribosomal Proteins lcsh:Medicine medicine.disease_cause Ribosome Inclusion bodies Article law.invention Escherichia coli Humans Recombinant Proteins 03 medical and health sciences 0302 clinical medicine law Ribosomal protein medicine Protein biosynthesis lcsh:Science Multidisciplinary Chemistry lcsh:R Biological techniques Fusion protein Chemical biology 030104 developmental biology Biochemistry Recombinant DNA lcsh:Q Thioredoxin 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Correddu, D, Montaño López, J D J, Vadakkedath, P G, Lai, A, Pernes, J I, Watson, P R & Leung, I K H 2019, ' An improved method for the heterologous production of soluble human ribosomal proteins in Escherichia coli ', Scientific Reports, vol. 9, no. 1, 8884 . https://doi.org/10.1038/s41598-019-45323-8 Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-45323-8 |
| Popis: | Human ribosomal proteins play important structural and functional roles in the ribosome and in protein synthesis. An efficient method to recombinantly produce and purify these proteins would enable their full characterisation. However, the production of human ribosomal proteins can be challenging. The only published method about the recombinant production of human ribosomal proteins involved the recovery of proteins from inclusion bodies, a process that is tedious and may lead to significant loss of yield. Herein, we explored the use of different Escherichia coli competent cells and fusion protein tags for the recombinant production of human ribosomal proteins. We found that, by using thioredoxin as a fusion protein, soluble ribosomal protein could be obtained directly from cell lysates, thus leading to an improved method to recombinantly produce these proteins. |
| Databáze: | OpenAIRE |
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