A Transcriptional Program Promotes Remodeling of GABAergic Synapses inCaenorhabditis elegans
| Autor: | David M. Miller, Mihail Sarov, Walter W. Walthall, Sarah C. Petersen, Janet E. Richmond, Joseph D. Watson |
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| Rok vydání: | 2011 |
| Předmět: |
Embryo
Nonmammalian Time Factors Movement Vesicle-Associated Membrane Protein 1 Green Fluorescent Proteins Chicken ovalbumin upstream promoter-transcription factor Receptors Cell Surface Article Animals Genetically Modified Receptors GABA medicine Animals RNA Messenger Caenorhabditis elegans Caenorhabditis elegans Proteins Transcription factor gamma-Aminobutyric Acid Homeodomain Proteins Motor Neurons Genetics Analysis of Variance biology Gene Expression Profiling General Neuroscience Motor neuron Microarray Analysis biology.organism_classification Cell biology medicine.anatomical_structure Spinal Cord Mutation Synapses Synaptic plasticity Homeobox GABAergic RNA Interference Neural development Transcription Factors |
| Zdroj: | The Journal of Neuroscience. 31:15362-15375 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.3181-11.2011 |
| Popis: | Although transcription factors are known to regulate synaptic plasticity, downstream genes that contribute to neural circuit remodeling are largely undefined. InCaenorhabditis elegans, GABAergic Dorsal D (DD) motor neuron synapses are relocated to new sites during larval development. This remodeling program is blocked in Ventral D (VD) GABAergic motor neurons by the COUP-TF (chicken ovalbumin upstream promoter transcription factor) homolog, UNC-55. We exploited this UNC-55 function to identify downstream synaptic remodeling genes that encode a diverse array of protein types including ion channels, cytoskeletal components, and transcription factors. We show that one of these targets, the Iroquois-like homeodomain protein, IRX-1, functions as a key regulator of remodeling in DD neurons. Our discovery ofirx-1as anunc-55-regulated target defines a transcriptional pathway that orchestrates an intricate synaptic remodeling program. Moreover, the well established roles of these conserved transcription factors in mammalian neural development suggest that a similar cascade may also control synaptic plasticity in more complex nervous systems. |
| Databáze: | OpenAIRE |
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