Expression of Angiopoietins and Angiogenic Signaling Pathway Molecules in Chronic Subdural Hematomas
| Autor: | Mika Ohmichi, Koji Osuka, Shigeru Miyachi, Yusuke Ohmichi, Munekazu Naito, Taiki Isaji, Takashi Nakano, Kenichiro Iwami |
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| Rok vydání: | 2020 |
| Předmět: |
Male
030506 rehabilitation Endothelium Angiogenesis Angiopoietin-2 Angiopoietin 03 medical and health sciences 0302 clinical medicine Chronic subdural hematoma Angiopoietin-1 Humans Medicine Aged Aged 80 and over biology business.industry Angiopoietins Middle Aged Receptor TIE-2 Angiopoietin receptor Embryonic stem cell surgical procedures operative medicine.anatomical_structure Hematoma Subdural Chronic cardiovascular system biology.protein Cancer research Female Neurology (clinical) Signal transduction 0305 other medical science business 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neurotrauma. 37:2493-2498 |
| ISSN: | 1557-9042 0897-7151 |
| DOI: | 10.1089/neu.2020.7042 |
| Popis: | Chronic subdural hematoma (CSDH) is an angiogenic disease that is involved with many inflammatory mediators. Tie2 is predominantly expressed in the embryonic endothelium and plays an important role in the maturation and stabilization of the vasculature. Angiopoietin (Ang)1 and Ang2 are well-known ligands of the Tie2 receptor. We examined the expression of Ang1 and Ang2 in CSDH fluid and the expression of Tie-2 receptor and components of the angiogenic signaling pathways in the outer membrane of CSDH. Twenty-five samples of CSDH fluid and eight samples of outer membrane of CSDH were included. The concentrations of Ang1 and Ang2 in the CSDH fluid were measured using enzyme-linked immunosorbent assay (ELISA) kits. The expression of Tie2, phosphoinositide 3-kinase (PI3K), protein kinase B (Akt) mechanistic target of rapamycin (mTOR), GβL, 70 kDa ribosomal protein S6 kinase (p70S6K), eukaryotic initiation factor 4E (eIF-4E), and β-actin was examined by a Western blot analysis. The expression of Tie2, Akt, and mTOR was also examined by immunohistochemistry. The concentration of Ang2 in CSDH fluid was significantly higher than that in the serum or cerebrospinal fluid (CSF), and also higher than that of Ang1 in CSDH fluid. Tie2, PI3K, Akt, mTOR, GβL, p70S6K, and eIF-4E were detected in all cases. In addition, Tie2, Akt, and mTOR were localized in the endothelial cells of vessels in the CSDH outer membrane. Our data suggest that Ang2, although not Ang1, in CSDH fluid promotes angiogenesis in endothelial cells through the Tie2 receptor. The Ang2/Tie2 signaling pathway might therefore be a useful therapeutic target for treating the growth of intractable CSDH. |
| Databáze: | OpenAIRE |
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