MicroRNAs related to cholesterol metabolism affected by vegetable diet in rainbow trout (Oncorhynchus mykiss) from control and selected lines

Autor: Mathilde Dupont-Nivet, Sandrine Skiba-Cassy, Jérôme Montfort, Edwige Quillet, Tengfei Zhu, Geneviève Corraze, Elisabeth Plagnes-Juan, Julien Bobe
Přispěvatelé: Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, ANR-13-ADAP-001 AGREENFISH, Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), This work was carried out within the framework of the AGREENFISH project (project ANR-13-ADAP-001) funded by the French National Research Agency (ANR). T. Zhu gratefully acknowledges the financial assistance provided by the China Scholarship Council (CSC, File No. 201406330071) for his doctoral fellowship., ANR-13-ADAP-0001,AGREENFISH,Adaptations aux transitions alimentaires en aquaculture: les caractériser et les favoriser(2013)
Rok vydání: 2019
Předmět:
food intake
[SDV]Life Sciences [q-bio]
adaptation
poisson
Aquaculture
salmonids
lipid metabolism
Food science
adaptation alimentaire
métabolisme du cholestérol
salmonidae
trout
alimentation
oncorhynchus mykiss
0303 health sciences
biology
04 agricultural and veterinary sciences
foie
Fish oil
rainbow trout
microRNAs
régime alimentaire
Trout
micro arn
expression des gènes
Aquatic Science
Selective breeding
03 medical and health sciences
Fish meal
rainbow
transcription factors
croissance animale
nutrition animale
signal de transduction
14. Life underwater
Salmonidae
030304 developmental biology
fish
apoptose
métabolisme lipidique
business.industry
Lipid metabolism
biology.organism_classification
plant-based diet
alimentation végétale
animal nutrition
cholesterol metabolism
040102 fisheries
0401 agriculture
forestry
and fisheries
Rainbow trout
liver function tests
business
facteur de transcription
animal growth
truite arc en ciel
Zdroj: Aquaculture
Aquaculture, Elsevier, 2019, 498, pp.132-142. ⟨10.1016/j.aquaculture.2018.08.058⟩
ISSN: 0044-8486
Popis: For the sustainable development of aquaculture, vegetable ingredients have been used to replace the traditional ingredients fishmeal and fish oil in aquafeeds. On the other hand, selective breeding has also been implemented at research level to obtain strains that are adapted to feeding on the plant-based diet. To better understand the underlying mechanisms prompting the adaptation to plant-based diets in fish, we investigated the hepatic expression of several microRNAs (miRNAs) that are involved in the post-transcriptional regulation of cholesterol and lipid metabolism at 8 h and 16 h after the last meal in two lines of rainbow trout: one selected for better adaptation to plant-based diets and the corresponding control line. Both groups were fed either a fishmeal and fish oil based diet or a 100% plant-based diet. Results showed that the expression of miR-33a in liver was greatly elevated in trout fed the plant-based diet, while the expression of miR-122 and miR-128 was much higher in the selected line at 8 h after the last meal regardless of the diet. Furthermore, our results indicated that some genes involved in immune processes (caspase 6 apoptosis-related cysteine peptidase like 2, casp6l2) and cAMP signal transduction (phosphodiesterase 4B cAMP-specific a, pde4ba) were also potentially regulated by miRNAs. They were newly identified as putative direct targets of miRNAs and affected in trout fed the plant-based diet. Though further investigations are still needed to establish a valid relationship between miRNAs and their target genes, our study found miR-33a, miR-122 and miR-128 as potential candidates for further study and provided new perspectives to understand the role of miRNAs in the selective breeding for adaptation to the plant-based diets.
Databáze: OpenAIRE
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