Production of reactive oxygen species is turned on and rapidly shut down in epithelial cells infected with Chlamydia trachomatis
| Autor: | Cédric Delevoye, Agathe Subtil, Gaelle Boncompain, Odile Kellermann, Benoit Schneider, Alice Dautry-Varsat |
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| Přispěvatelé: | Biologie des Interactions Cellulaires, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Laboratoire Cellules Souches, Signalisation et Prions, Université Paris Descartes - Paris 5 (UPD5), Pharmacologie, toxicologie et signalisation cellulaire (U747), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ERA-NET PathoGenoMics (ECIBUG), ANR-06-JCJC-0105,Chlam-hôte,Etude fonctionnelle des protéines sécrétées par la bactérie intracellulaire Chlamydia dans la cellule-hôte(2006), Biologie des Interactions Cellulaires (BIC), Institut Pasteur [Paris] - Centre National de la Recherche Scientifique (CNRS), Physiologie Cellulaire des Regulations Hormonales, Nutritionnelles et Pharmacologiques, Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS), ANR-06-JCJC-0105, Chlam-hôte, Etude fonctionnelle des protéines sécrétées par la bactérie intracellulaire Chlamydia dans la cellule-hôte(2006), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Immunology
Chlamydia trachomatis Biology medicine.disease_cause Microbiology 03 medical and health sciences NADPH oxidase complex Immunity medicine Humans Chlamydiaceae [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Reactive oxygen species Cellular Microbiology: Pathogen-Host Cell Molecular Interactions NADPH oxidase MESH: Humans MESH: Oxidative Stress 030306 microbiology MESH: Host-Pathogen Interactions NADPH Oxidases Epithelial Cells biology.organism_classification MESH: Reactive Oxygen Species/metabolism 3. Good health MESH: Hela Cells Oxidative Stress MESH: Chlamydia trachomatis/physiology Infectious Diseases [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology chemistry Host-Pathogen Interactions biology.protein Parasitology Reactive Oxygen Species MESH: NADPH Oxidase/metabolism Bacteria Oxidative stress HeLa Cells MESH: Epithelial Cells/microbiology |
| Zdroj: | Infection and Immunity Infection and Immunity, American Society for Microbiology, 2010, 78 (1), pp.80-7. ⟨10.1128/IAI.00725-09⟩ Infection and Immunity, American Society for Microbiology, 2010, 78 (1), pp.80-7. 〈10.1128/IAI.00725-09〉 Infection and Immunity, 2010, 78 (1), pp.80-7. ⟨10.1128/IAI.00725-09⟩ |
| ISSN: | 0019-9567 1098-5522 |
| DOI: | 10.1128/IAI.00725-09⟩ |
| Popis: | Reactive oxygen species (ROS) are many-faceted compounds involved in cell defense against pathogens, as well as in cell signaling. Their involvement in the response to infection in epithelial cells remains poorly documented. Here, we investigated the production of ROS during infection with Chlamydia trachomatis , a strict intracellular pathogen, in HeLa cells. C. trachomatis induced a transient increase in the ROS level within a few hours, followed by a return to basal level 9 hours after infection. At this time point, the host enzyme dedicated to ROS production, NADPH oxidase, could no longer be activated by external stimuli, such as interleukin-1β. In addition, Rac, a regulatory subunit of the NADPH oxidase complex, was relocated to the membrane of the compartment in which the bacteria develop, the inclusion, while other subunits were not. Altogether, these results indicate that C. trachomatis infection elicits the production of ROS and that the bacteria rapidly target the activity of NADPH oxidase to shut it down. Prevention of ROS production at the onset of the bacterial developmental cycle might delay the host response to infection. |
| Databáze: | OpenAIRE |
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