Retinoblastoma Function is a Better Indicator of Cellular Phenotype in Cultured Breast Adenocarcinoma Cells than Retinoblastoma Expression
| Autor: | Jeannine Botos, Roger Smith, Deborah T. Kochevar |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Molecular Sequence Data Breast Neoplasms Cell Cycle Proteins Adenocarcinoma Breast Adenocarcinoma Biology Retinoblastoma Protein Culture Media Serum-Free General Biochemistry Genetics and Molecular Biology Flow cytometry 03 medical and health sciences 0302 clinical medicine Cyclin D1 Breast cancer Tumor Cells Cultured medicine Humans Breast RNA Messenger Promoter Regions Genetic skin and connective tissue diseases E2F Regulation of gene expression Base Sequence medicine.diagnostic_test Retinoblastoma Cell Cycle Sequence Analysis DNA Flow Cytometry medicine.disease E2F Transcription Factors DNA-Binding Proteins Gene Expression Regulation Neoplastic Phenotype 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Cancer research Female Transcription Factors |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | Loss of or lowered retinoblastoma (Rb) expression has been included as a prognostic indicator in breast cancer. Low or no Rb expression is seen most commonly in high-grade breast adenocarcinomas, suggesting that a relationship may exist between loss of Rb and a less differentiated state, high proliferation rate, and high metastatic potential. In this study, we compared Rb function in two established breast adenocarcinoma cell lines, MCF-7 and MDA-MB-231, and in an established immortalized mammary epithelial cell line, MCF10A. Cells were synchronized in G0/G1 and were released for several durations, at which time total Rb protein, mRNA, and Rb/E2F/DNA complex formation were evaluated. Rb protein was significantly higher in the tumor cells than in MCF10A cells. However, Rb function was high for a longer duration in MCF10A cells as compared with MCF-7 and MDA-MB-231 cells. Our data support the general conclusion that Rb function, but not necessarily Rb protein, is lower in highly malignant breast adenocarcinoma cells as compared with lower grade tumor cells. These results emphasize the relevance of assessing Rb function over Rb protein. This is particularly important if Rb is to be used as a prognostic indicator for breast adenocarcinoma. |
| Databáze: | OpenAIRE |
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