Multi-omics Analyses of Starvation Responses Reveal a Central Role for Lipoprotein Metabolism in Acute Starvation Survival in C. elegans
| Autor: | Angus I. Lamond, Susanne Mandrup, Mark Larance, Richard R. Sprenger, Kathrine B. Dall, Anton Gartner, Eva Bang Harvald, Nils J. Færgeman, Christer S. Ejsing, Ronni Nielsen, Alejandro Brenes Murillo |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Proteomics Histology Lipoproteins Pathology and Forensic Medicine Transcriptome 03 medical and health sciences Gene Knockout Techniques Vitellogenins 0302 clinical medicine Lipid droplet medicine Journal Article Basic Helix-Loop-Helix Transcription Factors Animals Particle Size Caenorhabditis elegans Caenorhabditis elegans Proteins Transcription factor 2. Zero hunger Starvation Gene knockdown biology Sequence Analysis RNA Cell Biology Lipid Droplets biology.organism_classification Cell biology Metabolic pathway 030104 developmental biology Biochemistry RNA Interference medicine.symptom 030217 neurology & neurosurgery |
| Zdroj: | Harvald, E B, Sprenger, R R, Dall, K B, Ejsing, C S, Nielsen, R, Mandrup, S, Murillo, A B, Larance, M, Gartner, A, Lamond, A I & Færgeman, N J 2017, ' Multi-omics Analyses of Starvation Responses Reveal a Central Role for Lipoprotein Metabolism in Acute Starvation Survival in C. elegans ', Cell Systems, vol. 5, no. 1, pp. 38-52.e4 . https://doi.org/10.1016/j.cels.2017.06.004 |
| ISSN: | 2405-4712 |
| DOI: | 10.1016/j.cels.2017.06.004 |
| Popis: | Starvation causes comprehensive metabolic changes, which are still not fully understood. Here, we used quantitative proteomics and RNA sequencing to examine the temporal starvation responses in wild-type Caenorhabditis elegans and animals lacking the transcription factor HLH-30. Our findings show that starvation alters the abundance of hundreds of proteins and mRNAs in a temporal manner, many of which are involved in central metabolic pathways, including lipoprotein metabolism. We demonstrate that premature death of hlh-30 animals under starvation can be prevented by knockdown of either vit-1 or vit-5, encoding two different lipoproteins. We further show that the size and number of intestinal lipid droplets under starvation are altered in hlh-30 animals, which can be rescued by knockdown of vit-1. Taken together, this indicates that survival of hlh-30 animals under starvation is closely linked to regulation of intestinal lipid stores. We provide the most detailed poly-omic analysis of starvation responses to date, which serves as a resource for further mechanistic studies of starvation. |
| Databáze: | OpenAIRE |
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