Evidence of population structuring following population genetic analyses of Fasciola hepatica from Argentina
| Autor: | Nicola J. Beesley, Jorge Bruno Malandrini, Cesar Ivan Pruzzo, Severo Vázquez-Prieto, José D. Álvarez, Esperanza Paniagua, Jane E. Hodgkinson, Román Vilas, Florencio M. Ubeira, H. Solana, Elizabeth Attree, Oscar Jensen |
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| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía, Universidade de Santiago de Compostela. Departamento de Zooloxía, Xenética e Antropoloxía Física |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Fascioliasis Genotype Population genetics 030231 tropical medicine Population Argentina Zoology Population structure Clones Article 03 medical and health sciences 0302 clinical medicine Hepatica parasitic diseases Animals Humans Fasciola hepatica Parasite hosting education Evolutionary dynamics ComputingMethodologies_COMPUTERGRAPHICS education.field_of_study biology Genetic Variation biology.organism_classification Genetics Population 030104 developmental biology Infectious Diseases Genetic structure Parasitology |
| Zdroj: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela Universidad de Santiago de Compostela (USC) International Journal for Parasitology |
| Popis: | Graphical abstract Highlights • 320 Argentinian Fasciola hepatica were genotyped using a panel of microsatellites. • Overall there was high genotypic richness: 263 distinct genotypes were identified. • Population structuring of F. hepatica was evident across Argentina. • Within these sub-populations there is largely random mating. • Transmission of clonemates occurs: clonal parasites accounted for 26.6% of all parasites. Fasciola hepatica, the liver fluke, is a trematode parasite that causes disease of economic importance in livestock. As a zoonosis this parasite also poses a risk to human health in areas where it is endemic. Population genetic studies can reveal the mechanisms responsible for genetic structuring (non-panmixia) within parasite populations and provide valuable insights into population dynamics, which in turn enables theoretical predictions of evolutionary dynamics such as the evolution of drug resistance. Here we genotyped 320 F. hepatica collected from 14 definitive hosts from four provinces in Argentina. STRUCTURE analysis indicated three population clusters, and principal coordinate analysis confirmed this, showing population clustering across provinces. Similarly, pairwise FST values amongst all four provinces were significant, with standardised pairwise FST (F′ST) ranging from 0.0754 to 0.6327. Therefore, population genetic structure was evident across these four provinces in Argentina. However, there was no evidence of deviation from Hardy–Weinberg equilibrium, so it appears that within these sub-populations there is largely random mating. We identified 263 unique genotypes, which gave a clonal diversity of 82%. Parasites with identical genotypes, clones, accounted for 26.6% of the parasites studied and were found in 12 of the 14 hosts studied, suggesting some clonemate transmission. |
| Databáze: | OpenAIRE |
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