Endometrial stromal cell attachment and matrix homeostasis in abdominal wall endometriomas
Autor: | Haruta Mogami, R. Ann Word, Larry E. Word, Rodney T. Miller, H. Itoh, David E. Rogers, Laurice Bou Nemer |
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Rok vydání: | 2017 |
Předmět: |
Adult
0301 basic medicine Pathology medicine.medical_specialty Stromal cell MMP2 Endometriosis Endometrium Abdominal wall Young Adult 03 medical and health sciences 0302 clinical medicine Dermis Cell Adhesion medicine Homeostasis Humans RNA Messenger Cells Cultured Progesterone Endometrial Stromal Cell 030219 obstetrics & reproductive medicine business.industry Abdominal Wall Rehabilitation Obstetrics and Gynecology Middle Aged medicine.disease Extracellular Matrix 030104 developmental biology medicine.anatomical_structure Matrix Metalloproteinase 9 Reproductive Medicine Case-Control Studies Matrix Metalloproteinase 2 Immunohistochemistry Female Original Article Stromal Cells business |
Zdroj: | Human Reproduction. 33:280-291 |
ISSN: | 1460-2350 0268-1161 |
Popis: | STUDY QUESTION How does progesterone alter matrix remodeling in abdominal wall endometriomas compared with normal endometrium? SUMMARY ANSWER Progesterone may prevent attachment of endometrial cells to the abdominal wall, but does not ameliorate abnormal stromal cell responses of abdominal wall endometriomas. WHAT IS KNOWN ALREADY Menstruation is a tightly orchestrated physiologic event in which steroid hormones and inflammatory cells cooperatively initiate shedding of the endometrium. Abdominal wall endometriomas represent a unique form of endometriosis in which endometrial cells inoculate fascia or dermis at the time of obstetrical or gynecologic surgery. Invasion of endometrium into ectopic sites requires matrix metalloproteinases (MMPs) for tissue remodeling but endometrium is not shed externally. STUDY DESIGN SIZE, DURATION Observational study in 14 cases and 19 controls. PARTICIPANTS /MATERIALS, SETTING, METHODS Tissues and stromal cells isolated from 14 abdominal wall endometriomas were compared with 19 normal cycling endometrium using immunohistochemistry, quantitative PCR, gelatin zymography and cell attachment assays. P values < 0.05 were considered significant and experiments were repeated in at least three different cell preps to provide scientific rigor to the conclusions. MAIN RESULTS AND THE ROLE OF CHANCE The results indicate that MMP2 and MMP9 are not increased by TGFβ1 in endometrioma stromal cells. Although progesterone prevents attachment of endometrioma cells to matrix components of the abdominal wall, it does not ameliorate these abnormal stromal cell responses to TGFβ1. LARGE SCALE DATA N/A. LIMITATIONS REASONS FOR CAUTION Endometriomas were collected from women identified pre-operatively. Not all endometriomas were collected. Stromal cells from normal endometrium were from different patients, not women undergoing endometrioma resection. WIDER IMPLICATIONS OF THE FINDINGS This work provides insight into the mechanisms by which progesterone may prevent abdominal wall endometriomas but, once established, are refractory to progesterone treatment. STUDY FUNDING/COMPETING INTEREST(S) Tissue acquisition was supported by NIH P01HD087150. Authors have no competing interests. |
Databáze: | OpenAIRE |
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