Colicin activity and abortive infection of T5 bacteriophage in Escherichia coli (ColIb)
| Autor: | G B Dunn, D H Duckworth, S K Walia, T Pinkerton, K Rose |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1981 |
| Předmět: |
inorganic chemicals
Kanamycin Resistance Plating efficiency Immunology Mutant Bacteriocin Plasmids Colicins Biology medicine.disease_cause Microbiology Bacteriophage Plasmid Virology medicine Escherichia coli technology industry and agriculture biochemical phenomena metabolism and nutrition biology.organism_classification Insect Science Colicin Mutation DNA Transposable Elements bacteria lipids (amino acids peptides and proteins) T-Phages Research Article Plasmids |
| Popis: | We performed three types of experiments to test the hypothesis that abortive infection of T5 bacteriophage in Escherichia coli (ColIb+) is due to internally released colicin. (i) We measured the sensitivity of cells to colicin under a variety of conditions and then looked at the plating efficiency of T5 in ColIb+ cells under these same conditions. Cells grown at 42 degrees C or with hexanol had a reduced sensitivity to externally added colicin and an increased efficiency for T5 when the ColIb plasmid was present in the infected cells. Phage growth was far from normal, however. (ii) We measured the colicin sensitivity of a mutant bacterium that grew T5 normally even in the presence of the ColIb plasmid and measured the plating efficiency of T5 on another mutant that was colicin tolerant. Here again, the correlation between colicin activity and inhibition of phage replication was not complete. (iii) We looked for colicin-negative plasmid mutants and tested the ability of cells containing these plasmids to support the growth of T5. These experiments used Tn5, a kanamycin resistance transposon, as the mutagen. All possible combinations of colicin production and phage inhibition were found, including mutants that produced no colicin but still inhibited phage production. |
| Databáze: | OpenAIRE |
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