Transmission experiments support clade-level differences in the transmission and pathogenicity of Cambodian influenza A/H5N1 viruses
Autor: | Sopheaktra Ros, Richard J. Webby, Trushar Jeevan, A. E. Metlin, Songha Tok, Paul F. Horwood, Sareth Rith, Phalla Y, Thomas P. Fabrizio, Erik A. Karlsson, Philippe Buchy, Annika Suttie, Patrick Seiler, Philippe Dussart, Srey Viseth Horm |
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Přispěvatelé: | Unité de Virologie / Virology Unit [Phnom Penh], Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), James Cook University (JCU), St Jude Children's Research Hospital, Federation University [Churchill, Australia], GlaxoSmithKline Vaccines [Singapore], GlaxoSmithKline [Headquarters, London, UK] (GSK), The study was partially funded by the US Agency for International Development [grant number AID-442-G-14-00005]. This work was also partially supported by the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under CEIRS contract no. HHSN272201400006C. |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Epidemiology viruses MESH: Virulence medicine.disease_cause MESH: Ferrets Drug Discovery ducks MESH: Ducks MESH: Animals Clade MESH: Phylogeny MESH: Orthomyxoviridae Infections Phylogeny Virulence Transmission (medicine) poultry MESH: Influenza Human transmission MESH: Chickens virus diseases General Medicine H5N1 Infectious Diseases [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Enzootic Original Article Cambodia Research Article MESH: Influenza A Virus H5N1 Subtype 030106 microbiology Immunology Biology Microbiology Virus 03 medical and health sciences Orthomyxoviridae Infections Species Specificity MESH: Influenza in Birds Virology Influenza Human medicine Animals Humans MESH: Species Specificity MESH: Humans Influenza A Virus H5N1 Subtype avian MESH: Cambodia Influenza A/H5N1 Pathogenicity Influenza A virus subtype H5N1 Influenza 030104 developmental biology Influenza in Birds Parasitology Chickens ferrets |
Zdroj: | Emerging microbes & infections Emerging microbes & infections, Earliest : Springer-Nature ; Latest : Taylor & Francis, 2020, 9 (1), pp.1702-1711. ⟨10.1080/22221751.2020.1792353⟩ Emerging Microbes & Infections article-version (VoR) Version of Record |
ISSN: | 2222-1751 |
Popis: | International audience; Influenza A/H5N1 has circulated in Asia since 2003 and is now enzootic in many countries in that region. In Cambodia, the virus has circulated since 2004 and has intermittently infected humans. During this period, we have noted differences in the rate of infections in humans, potentially associated with the circulation of different viral clades. In particular, a reassortant clade 1.1.2 virus emerged in early 2013 and was associated with a dramatic increase in infections of humans (34 cases) until it was replaced by a clade 2.3.2.1c virus in early 2014. In contrast, only one infection of a human has been reported in the 6 years since the clade 2.3.2.1c virus became the dominant circulating virus. We selected three viruses to represent the main viral clades that have circulated in Cambodia (clade 1.1.2, clade 1.1.2 reassortant, and clade 2.3.2.1c), and we conducted experiments to assess the virulence and transmissibility of these viruses in avian (chicken, duck) and mammalian (ferret) models. Our results suggest that the clade 2.3.2.1c virus is more "avian-like," with high virulence in both ducks and chickens, but there is no evidence of aerosol transmission of the virus from ducks to ferrets. In contrast, the two clade 1 viruses were less virulent in experimentally infected and contact ducks. However, evidence of chicken-to-ferret aerosol transmission was observed for both clade 1 viruses. The transmission experiments provide insights into clade-level differences that might explain the variation in A/H5N1 infections of humans observed in Cambodia and other settings. |
Databáze: | OpenAIRE |
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