Metal-Organic Framework Encapsulated Whole-Cell Vaccines Enhance Humoral Immunity against Bacterial Infection
| Autor: | Molly A. Ingersoll, Sundharamani Venkitapathi, Thomas Howlett, Nicole J. De Nisco, Michael A. Luzuriaga, Olivia R. Brohlin, Kavya Veera, Fabian C. Herbert, Jashkaran Gadhvi, Michael D. Burton, Arezoo Shahrivarkevishahi, Candace E. Benjamin, Ryanne Ehrman, Jeremiah J. Gassensmith, Yalini H. Wijesundara, Sarah Popal |
|---|---|
| Přispěvatelé: | University of Texas at Dallas [Richardson] (UT Dallas), Département d'Immunologie - Department of Immunology, Institut Pasteur [Paris], Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité) |
| Rok vydání: | 2020 |
| Předmět: |
medicine.drug_class
Antibiotics General Physics and Astronomy 02 engineering and technology medicine.disease_cause Microbiology Mice 03 medical and health sciences Immune system Antigen vaccine Escherichia coli medicine Animals General Materials Science Antigens Metal-Organic Frameworks MOF 030304 developmental biology Vaccines 0303 health sciences biology Immunogenicity Vaccination General Engineering E. coli Pathogenic bacteria Bacterial Infections 021001 nanoscience & nanotechnology biology.organism_classification Immunity Humoral 3. Good health germinal center Humoral immunity [SDV.IMM]Life Sciences [q-bio]/Immunology whole cell vaccine urinary tract infection 0210 nano-technology Bacteria |
| Zdroj: | ACS Nano ACS Nano, American Chemical Society, 2021, ⟨10.1021/acsnano.1c03092⟩ ACS Nano, 2021, ⟨10.1021/acsnano.1c03092⟩ |
| ISSN: | 1936-0851 |
| DOI: | 10.1101/2020.06.14.148452 |
| Popis: | The increasing rate of resistance of bacterial infection against antibiotics requires next generation approaches to fight potential pandemic spread. The development of vaccines against pathogenic bacteria has been difficult owing, in part, to the genetic diversity of bacteria. Hence, there are many potential target antigens and little a priori knowledge of which antigen/s will elicit protective immunity. The painstaking process of selecting appropriate antigens could be avoided with whole-cell bacteria; however, whole-cell formulations typically fail to produce long-term and durable immune responses. These complications are one reason why no vaccine against any type of pathogenic E. coli has been successfully clinically translated. As a proof of principle, we demonstrate a method to enhance the immunogenicity of a model pathogenic E. coli strain by forming a slow releasing depot. The E. coli strain CFT073 was biomimetically mineralized within a metal-organic framework (MOF). This process encapsulates the bacteria within 30 minutes in water and at ambient temperatures. Vaccination with this new formulation substantially enhances antibody production and results in significantly enhanced survival in a mouse model of bacteremia compared to standard inactivated formulations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|