Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes
| Autor: | Shuntaro Yamashita, Mai Kuroda, Yoshinori Katakura, Haruka Matsuo, Kuniyoshi Shimizu, Zhao Chong, Hira Kaji Manandhar, Gopal Prasad Parajuli |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
animal structures Short Communication p38 mitogen-activated protein kinases Clinical Biochemistry Biomedical Engineering Cellular senescence Bioengineering 03 medical and health sciences Trametes versicolor Mushroom integumentary system biology Chemistry fungi food and beverages Ultraviolet b Cell Biology biology.organism_classification Cell biology enzymes and coenzymes (carbohydrates) Cytosol HaCaT 030104 developmental biology Phosphorylation Biotechnology |
| Zdroj: | Cytotechnology. 70:1001-1008 |
| ISSN: | 1573-0778 0920-9069 |
| Popis: | Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated β-galactosidase, p21, p16, phosphorylated p38, and γH2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10616-018-0229-1) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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