Contrasting cytotoxic mechanisms of similar antitumour diaziridinylbenzoquinones
| Autor: | John Butler, A Dzielendziak, Timothy H Ward, J S Lea, Brigid M Hoey |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Alkylating Agents
Free Radicals Cell Survival Aziridines Covalent binding Antineoplastic Agents Biochemistry chemistry.chemical_compound Drug Stability Benzoquinones Cytotoxic T cell Animals Humans Cytotoxicity Chromatography High Pressure Liquid Hplc analysis Aqueous medium Electron Spin Resonance Spectroscopy DNA Hydrogen-Ion Concentration Kinetics chemistry Oxidation-Reduction Function (biology) K562 cells |
| Zdroj: | Free radical research communications. 8(4-6) |
| ISSN: | 8755-0199 |
| Popis: | The mechanisms of cytotoxicity of the antitumour diaziridinylbenzoquinones, AZQ and BZQ. have been investigated. HPLC analysis has been used to study the products as well as the rate of decomposition of acid-assisted ring-opening in aqueous medium as a function of pH. Microconcentrators with a molecular weight cutoff of 30 kDa were utilised to study the covalent binding of both compounds to calf thymus DNA. Radical production of both compounds in K562 cell incubations was followed using ESR and their uptake into K562 cells was monitored using radiolabelled compounds. The results show that these two diaziridinylbenzoquinones. although very similar in structure, have diverse mechanisms of cytotoxicity. The implications of these findings are discussed in the light of antitumour action |
| Databáze: | OpenAIRE |
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