A glycoprotein with anti-inflammatory properties secreted by an Aspergillus nidulans modified strain
| Autor: | P. J. Spencer, Catarina Teixeira, J. C. F. Queiroz, T. M. A. D. Zucchi, I. Lebrun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
glycoprotein
Glycosylation lcsh:Arctic medicine. Tropical medicine Lipopolysaccharide medicine.drug_class lcsh:RC955-962 Toxicology Anti-inflammatory Aspergillus nidulans chemistry.chemical_compound Valine lcsh:RA1190-1270 lcsh:Zoology medicine lcsh:QL1-991 anti-inflammatory activity lcsh:Toxicology. Poisons Gel electrophoresis chemistry.chemical_classification biology biology.organism_classification Molecular biology protein purification and characterization Amino acid Infectious Diseases chemistry Biochemistry Animal Science and Zoology Parasitology Glycoprotein |
| Zdroj: | Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 13, Iss 2, Pp 527-543 (2007) Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 13, Issue: 2, Pages: 527-543, Published: 2007 Journal of Venomous Animals and Toxins including Tropical Diseases v.13 n.2 2007 The Journal of venomous animals and toxins including tropical diseases Universidade Estadual Paulista (UNESP) instacron:UNESP Scopus-Elsevier |
| ISSN: | 1678-9199 |
| Popis: | Total RNA from lipopolysaccharide (LPS)-stimulated rat macrophages used to treat protoplasts from an Aspergillus nidulans strain originated the RT2 regenerated strain, whose culture supernatant showed anti-inflammatory activity in Wistar rats. The protein fraction presenting such anti-inflammatory activity was purified and biochemically identified. The screening of the fraction responsible for such anti-inflammatory property was performed by evaluating the inhibition of carrageenan-induced paw edema in male Swiss mice. Biochemical analyses of the anti-inflammatory protein used chromatography, carbohydrates quantification of the protein sample, amino acids content analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Total sugar quantification revealed 32% glycosylation of the protein fraction. Amino acid analysis of such fraction showed a peculiar pattern presenting 29% valine. SDS-PAGE revealed that the protein sample is pure and its molecular weight is about 40kDa. Intravenous injection of the isolated substance into mice significantly inhibited carrageenan-induced paw edema. The isolated glycoprotein decreased carrageenan-induced paw edema in a prostaglandin-dependent phase, suggesting an inhibitory effect of the isolated glycoprotein on prostaglandin synthesis. |
| Databáze: | OpenAIRE |
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