Cytokine tear film profile determination in eyes of healthy dogs and those with inflammatory periocular and skin disorders
| Autor: | C.C. Liu, P.S. Martinez, C.M. Pucheu, R.T. Carter |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Eye Diseases 040301 veterinary sciences Cytokine profile medicine.medical_treatment Immunology Inflammation Disease Biology Eye Skin Diseases 0403 veterinary science 03 medical and health sciences Dogs medicine Animals Dog Diseases 030304 developmental biology 0303 health sciences General Veterinary Interleukin-6 Interleukin-8 04 agricultural and veterinary sciences Dermatology eye diseases Serum cytokine Cytokine Tears Tumor Necrosis Factors Cytokines Interleukin-2 Female sense organs medicine.symptom |
| Zdroj: | Veterinary Immunology and Immunopathology. 221:110012 |
| ISSN: | 0165-2427 |
| Popis: | Alterations in serum cytokine levels and profiles have been reported in association with a variety of disease conditions (e.g., allergic, immune-mediated, etc.) in both humans and animals. In comparison to serum cytokine measurements, tear cytokine measurements might be expected to more accurately reflect the inflammatory milieu associated with periocular disease. The purpose of this study was to use a multiplexed assay to compare the cytokine profile of tears in healthy dogs to those with inflammatory skin and periocular disease. We were able to detect IL-2, IL-6, IL-8, and TNF-α in47 % of tear samples from both healthy canine patients and those with inflammatory dermatologic disease (with or without concurrent periocular involvement). In contrast, IL-7, IL-10 and IFN-γ were rarely detected. Dogs with both dermatologic and periocular disease (but not dermatologic disease alone) had higher levels of IL-8 (P 0.001, P 0.05, respectively) relative to healthy dogs. Patients with concurrent dermatologic and periocular disease also demonstrated significantly greater variability in IL-8 concentrations between eyes than did healthy dogs (P 0.0001). Our findings suggest that tear cytokine analysis may prove to be a useful tool to investigate the role and interactions of the local ocular immune response in patients with inflammatory periocular disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect