Purification Methodology for Viable and Infective Plasmodium vivax Gametocytes That Is Compatible with Transmission-Blocking Assays
| Autor: | Fabio T. M. Costa, Paula Brelas de Brito, Wuelton Marcelo Monteiro, Letusa Albrecht, Stefanie C. P. Lopes, Omaira Vera, Paulo F. P. Pimenta, Marcus V. G. Lacerda, Keillen M Martins-Campos |
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| Rok vydání: | 2015 |
| Předmět: |
Pharmacology
Analytical Procedures biology Plasmodium vivax Plasmodium falciparum biology.organism_classification medicine.disease Virology Transmission blocking Peripheral blood Infectious Diseases Vivax malaria parasitic diseases Gametocyte medicine Malaria Vivax Parasite hosting Humans Pharmacology (medical) Malaria Falciparum Malaria |
| Zdroj: | Antimicrobial agents and chemotherapy. 59(10) |
| ISSN: | 1098-6596 |
| Popis: | Significant progress toward the control of malaria has been achieved, especially regarding Plasmodium falciparum infections. However, the unique biology of Plasmodium vivax hampers current control strategies. The early appearance of P. vivax gametocytes in the peripheral blood and the impossibility of culturing this parasite are major drawbacks. Using blood samples from 40 P. vivax -infected patients, we describe here a methodology to purify viable gametocytes and further infect anophelines. This method opens new avenues to validate transmission-blocking strategies. |
| Databáze: | OpenAIRE |
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