Establishment and characterization of a human intrahepatic cholangiocarcinoma cell line derived from an Italian patient

Autor: Giuliana Cavalloni, Carmine Dell'Aglio, Francesco Leone, Chiara Varamo, Giovanna Chiorino, Laura Casorzo, Massimo Aglietta, Paola Bernabei, Caterina Peraldo-Neia
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Homeobox protein NANOG
Pathology
medicine.medical_specialty
Cancer Research
Carcinogenicity Tests
New cell line
DNA Mutational Analysis
Population
Mutation
Missense
Mice
SCID
medicine.disease_cause
Cholangiocarcinoma
Proto-Oncogene Proteins p21(ras)
03 medical and health sciences
0302 clinical medicine
SOX2
In vitro model
Cell Movement
Mice
Inbred NOD
In vivo
Cell Line
Tumor
Animals
Humans
Medicine
Intrahepatic cholangiocarcinoma
education
Intrahepatic Cholangiocarcinoma
Comparative Genomic Hybridization
education.field_of_study
Mutation
business.industry
CD24
General Medicine
Middle Aged
medicine.disease
Primary tumor
030104 developmental biology
Bile Duct Neoplasms
Italy
030220 oncology & carcinogenesis
Cancer research
Original Article
Female
business
Neoplasm Transplantation
Zdroj: Tumour Biology
Popis: Biliary tract carcinoma is a rare malignancy with multiple causes, which underlie the different genetic and molecular profiles. Cancer cell lines are affordable models, reflecting the characteristics of the tumor of origin. They represent useful tools to identify molecular targets for treatment. Here, we established and characterized from biological, molecular, and genetic point of view, an Italian intrahepatic cholangiocarcinoma cell line (ICC), the MT-CHC01. MT-CHC01 cells were isolated from a tumor-derived xenograft. Immunophenotypical characterization was evaluated both at early and after stabilization passages. In vitro biological, genetic, and molecular features were also investigated. In vivo tumorigenicity was assessed in NOD/SCID mice. MT-CHC01cells retain epithelial cell markers, EPCAM, CK7, and CK19, and some stemness and pluripotency markers, i.e., SOX2, Nanog, CD49f/integrin-α6, CD24, PDX1, FOXA2, and CD133. They grow as a monolayer, with a population double time of about 40 h; they show a low migration and invasion potential. In low attachment conditions, they are able to form spheres and to growth in anchorage-independent manner. After subcutaneous injection, they retain in vivo tumorigenicity; the expression of biliary markers as CA19-9 and CEA were maintained from primary tumor. The karyotype is highly complex, with a hypotriploid to hypertriploid modal number (3n+/−) (52 to 77 chromosomes); low level of HER2 gene amplification, TP53 deletion, gain of AURKA were identified; K-RAS G12D mutation were maintained from primary tumor to MT-CHC01 cells. We established the first ICC cell line derived from an Italian patient. It will help to study either the biology of this tumor or to test drugs both in vitro and in vivo. Electronic supplementary material The online version of this article (doi:10.1007/s13277-015-4215-3) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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