Strategy for erythroid differentiation in ex vivo cultures: Lentiviral genetic modification of human hematopoietic stem cells with erythropoietin gene

Autor: Marina Etcheverrigaray, Luisina Anabel Cappellino, Ricardo Kratje, Claudio Prieto
Rok vydání: 2017
Předmět:
Zdroj: Journal of Bioscience and Bioengineering. 124:591-598
ISSN: 1389-1723
DOI: 10.1016/j.jbiosc.2017.06.005
Popis: If cultured in appropriate conditions, such as supplementing culture media with costly cytokines and growth factors, hematopoietic stem/progenitor cells (HSPCs) from different origins have shown to be an adequate source of erythroid cells. This requirement turns erythroid cells production into a complicated process to be scaled-up for future applications. The aim of our work was to genetically modify HSPCs with human erythropoietin (hEPO) sequence by lentiviral transgenesis in order for cells to secrete the hormone into the culture medium. Initially, we evaluated erythroid differentiation in colony forming units (CFU) assays and further analyzed cell expansion and erythroid differentiation throughout time in suspension cultures by flow cytometry and May-Grünwald-Giemsa staining. Additionally, we studied hEPO production and its isoforms profile. The different assessment approaches demonstrated erythroid differentiation, which was attributed to the hEPO secreted by the HSPCs. Our data demonstrate that it is possible to develop culture systems in which recombinant HSPCs are self-suppliers of hEPO. This feature makes our strategy attractive to be applied in biotechnological production processes of erythroid cells that are currently under development. Fil: Cappellino, Luisina Anabel. Universidad Nacional del Litoral; Argentina Fil: Kratje, Ricardo Bertoldo. Universidad Nacional del Litoral; Argentina Fil: Etcheverrigaray, Marina. Universidad Nacional del Litoral; Argentina Fil: Prieto, Claudio. Universidad Nacional del Litoral; Argentina
Databáze: OpenAIRE
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