Contribution of serotype-specific IgG concentration, IgG subclasses and relative antibody avidity to opsonophagocytic activity against Streptococcus pneumoniae
| Autor: | V Jäntti, Eskola J, Helena Käyhty, M Voutilainen, Anttila M |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Serotype Time Factors Immunology Dose-Response Relationship Immunologic chemical and pharmacologic phenomena Biology medicine.disease_cause Active immunization Microbiology Serology Immunoenzyme Techniques Phagocytosis Antibody Specificity Streptococcus pneumoniae medicine Humans Immunology and Allergy Avidity Serotyping Bacterial Capsules Age Factors Infant Original Articles Opsonin Proteins Antibodies Bacterial Isotype Antibody opsonization Immunoglobulin G Bacterial Vaccines biology.protein Biological Assay Antibody |
| Zdroj: | Clinical and Experimental Immunology. 118:402-407 |
| ISSN: | 1365-2249 0009-9104 |
| Popis: | SUMMARY The contribution of serotype-specific IgG concentration, subclasses, and avidity to opsonophagocytic activity (OPA) against Streptococcus pneumoniae (Pnc) was evaluated in sera of adults and infants immunized with different pneumococcal vaccines. Antibody concentrations and avidities were measured by enzymeimmunoassay (EIA) and OPAs by killing assay of Pnc. The most important factor contributing positively to OPA was the specific IgG level. In infants, a tendency to negative correlation was found between the concentration needed for killing of bacteria and avidity, suggesting that less antibodies of high rather than low avidity were required for killing. No such correlation was seen in adults. However, in adults the avidity was high already before vaccination and the variation was narrow. Thus, avidity was probably not a limiting factor influencing OPA. The effect of IgG2/IgG1 ratio on OPA was mostly negative but insignificant. |
| Databáze: | OpenAIRE |
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