Total flavonoids of Herba Epimedii improves osteogenesis and inhibits osteoclastogenesis of human mesenchymal stem cells
| Autor: | Ping-Chung Leung, Chu-yan Chan, Jin-fang Zhang, Guo Li, Yaou Zhang, Qi Dong, Chun-ling Meng, Ming-Liang He, Hsiang-Fu Kung |
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| Rok vydání: | 2009 |
| Předmět: |
Phosphoric monoester hydrolases
Osteoporosis Osteoclasts Pharmaceutical Science Immunophenotyping Osteogenesis Osteoclast Drug Discovery medicine Humans RNA Messenger Cells Cultured DNA Primers Flavonoids Pharmacology Bone mineral Base Sequence biology Reverse Transcriptase Polymerase Chain Reaction Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells medicine.disease medicine.anatomical_structure Complementary and alternative medicine Biochemistry RANKL biology.protein Cancer research Molecular Medicine Alkaline phosphatase Female Bone marrow Drugs Chinese Herbal |
| Zdroj: | Phytomedicine. 16:521-529 |
| ISSN: | 0944-7113 |
| DOI: | 10.1016/j.phymed.2009.01.003 |
| Popis: | Introduction In China Herba Epimedii is one of the most common herbs that could be prescribed for treating osteoporosis. It is known to increase the overall mineral content, therefore, to promote bone formation and to increase lumbar bone mineral density (BMD). The present study was aimed at investigating the effect of flavonoids of Herba Epimedii (HEF) on osteogenesis in human MSCs. Methods The human bone marrow-derived MSCs (BM-MSCs) were isolated and their osteogenic differentiation was evaluated by their alkaline phosphatase (ALP) activities and level of mineralization. After treating with total flavonoids during osteogenic differentiation process, differential mRNA expression was examined by RT-PCR. Results The total time needed for osteogenic differentiation of BM-MSCs was significantly shortened by adding HEF. Up-regulation of mRNA expression by HEF was observed for several marker genes and osteogenic regulators. HEF was also found to inhibit osteoclastogenesis of MSCs by enhancing the ratio OPG/RANKL. Conclusions Our study demonstrated that the HEF could improve osteogenic differentiation and inhibit the osteoclast differentiation of BM-MSCs concurrently. |
| Databáze: | OpenAIRE |
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