Secretory proteins compete for production in the mammary gland of transgenic mice
| Autor: | Anthony John Clark, Colin J. Wilde, A Springbett, M McClenaghan, R M Wallace |
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| Rok vydání: | 1995 |
| Předmět: |
Genetically modified mouse
Transgene Gene Expression Mice Transgenic Endogeny Lactoglobulins Biology Biochemistry Mice Mammary Glands Animal Casein Gene expression Animals RNA Messenger Molecular Biology Messenger RNA Sheep food and beverages Cell Biology Milk Proteins Molecular biology Kinetics Milk Secretory protein biology.protein Electrophoresis Polyacrylamide Gel Female Whey Acidic Protein Research Article |
| Zdroj: | Biochemical Journal. 310:637-641 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3100637 |
| Popis: | To explore the possibility that genes might compete for expression, we have studied transgenic mice producing high levels of the sheep milk protein, beta-lactoglobulin (BLG), in the mammary gland. Mice carrying one or more transgene loci expressed BLG in milk at levels ranging from 7 to 33 mg/ml. The effects of BLG synthesis on the levels of endogenous milk gene expression were examined. No significant increase in total milk protein concentration was recorded even in mice expressing the largest amounts of BLG. Measurement of individual milk proteins showed that transgene protein was manufactured at the expense of host protein synthesized in the gland. Whey acidic protein production was more suppressed than casein production. Suppression of endogenous proteins was matched by a reduction in the corresponding steady-state mRNA levels; in double-transgenic mice, which expressed the largest amounts of BLG, beta-casein and whey acidic protein mRNA populations were reduced to 75 and 56% of control levels respectively. We demonstrate that an exogenous gene competes effectively for expression with endogenous genes. Possible mechanisms of competition are discussed. |
| Databáze: | OpenAIRE |
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