Phenotypic methods for screening carbapenem-resistant Enterobacteriaceae and assessment of their antimicrobial susceptibility profile
| Autor: | Roberta Filipini Rampelotto, Silvana Oliveira dos Santos, Vinícius Victor Lorenzoni, Manfredo Hörner, Danielly da Costa Silva, Juliana Raquel da Silva Damer, Rosmari Hörner |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Klebsiella pneumoniae Phenotypic screening 030106 microbiology Carbapenem-resistant enterobacteriaceae Drug resistance Microbial Sensitivity Tests Biology beta-Lactam Resistance Microbiology Hospitals University 03 medical and health sciences Cloxacillin Enterobacteriaceae medicine Humans Klebsiella pneumonia Carbapenemens medicine.disease biology.organism_classification Antimicrobial Anti-Bacterial Agents 030104 developmental biology Infectious Diseases Phenotype Carbapenems Parasitology Microbial sensitive tests medicine.drug |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical, Volume: 50, Issue: 2, Pages: 173-178, Published: MAR 2017 Revista da Sociedade Brasileira de Medicina Tropical, Vol 50, Iss 2, Pp 173-178 Revista da Sociedade Brasileira de Medicina Tropical v.50 n.2 2017 Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
| ISSN: | 1678-9849 |
| Popis: | INTRODUCTION: In this study, we used phenotypic methods to screen carbapenem-resistant Enterobacteriaceae (CREs) and evaluated their antimicrobial sensitivity profile. METHODS: One hundred and seventy-eight CREs were isolated at a university hospital in south Brazil in a one-year period. Samples were assessed using disk diffusion tests with inhibitors of β-lactamases such as phenylboronic acid (AFB), cloxacillin (CLOXA), and ethylenediaminetetraacetic acid (EDTA). Strains with differences in zone diameters ≥ 5mm for disks supplemented or not were considered producers of carbapenemases. RESULTS: Klebsiella pneumoniae was the most prevalent CRE, which appeared in 80.3% cases (n = 143). Among clinical materials, the rectal swab was responsible for 43.4% of the isolations (n = 62), followed by urine (18.9%; n = 27). Among the CREs identified in this study, the growth of 56.7% (n = 101) isolates, which were putative producers of Klebsiella pneumoniae carbapenemase (KPC), were inhibited by AFB, whereas 7.3% (n = 13) isolates were inhibited by both AFB and CLOXA and were considered as putative producers of plasmid-mediated AmpC; approximately 3.4% (n = 6) were inhibited by EDTA, which possibly produced metallo-β-lactamase. Lastly, 32.6% (n = 58) cases showed negative results for AFB, CLOXA, and EDTA sensitivity, and represented another class of β-lactamases and/or mechanism of resistance. CONCLUSIONS: Phenotypic screening of CREs is important for clinical laboratories that monitor outbreaks of resistant microbes. Phenotypic tests that use carbapenemase inhibitors and enhancers such as AFB, CLOXA, and EDTA are necessary since they are good screening methods for the detection of carbapenemases. |
| Databáze: | OpenAIRE |
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