Light contamination in stable isotope-labelled internal peptide standards is frequent and a potential source of false discovery and quantitation error in proteomics

Autor:	Mogjiborahman Salek, Jonas D. Förster, Wolf-Dieter Lehmann, Angelika B. Riemer
Rok vydání:	2022
Předmět:	Proteomics Isotopes Isotope Labeling Reference Standards Peptides Biochemistry Mass Spectrometry Analytical Chemistry
Zdroj:	Analytical and Bioanalytical Chemistry. 414:2545-2552
ISSN:	1618-2650 1618-2642
DOI:	10.1007/s00216-022-03931-w
Popis:	In mass spectrometry-based proteomics, heavy internal standards are used to validate target peptide detections and to calibrate peptide quantitation. Here, we report light contamination present in heavy labelled synthetic peptides of high isotopic enrichment. Application of such peptides as assay-internal standards potentially compromises the detection and quantitation especially of low abundant cellular peptides. Therefore, it is important to adopt guidelines to prevent false-positive identifications of endogenous light peptides as well as errors in their quantitation from biological samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3013f9e3d2e725ce1e4968aec093158 https://doi.org/10.1007/s00216-022-03931-w Zobrazit plný text záznamu Plný text ve formátu PDF