Vitamin C Stimulates Epidermal Ceramide Production by Regulating Its Metabolic Enzymes
Autor: | Shivtaj Mann, Kyong-Oh Shin, Hye Jeong Yun, Kyungho Park, Yong-Moon Lee, Kun Pyo Kim, Yunhi Cho |
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Rok vydání: | 2015 |
Předmět: |
Vitamin
Ceramide metabolic enzymes Ceramide Epidermal barrier chemistry.chemical_element Calcium Biology Biochemistry chemistry.chemical_compound Drug Discovery medicine Vitamin C Ceramide synthase Pharmacology Keratinocyte differentiation Serine C-palmitoyltransferase Lipid signaling medicine.anatomical_structure chemistry Molecular Medicine Original Article Keratinocyte Sphingomyelin |
Zdroj: | Biomolecules & Therapeutics |
ISSN: | 1976-9148 2005-4483 |
DOI: | 10.4062/biomolther.2015.044 |
Popis: | Ceramide is the most abundant lipid in the epidermis and plays a critical role in maintaining epidermal barrier function. Overall ceramide content in keratinocyte increases in parallel with differentiation, which is initiated by supplementation of calcium and/or vitamin C. However, the role of metabolic enzymes responsible for ceramide generation in response to vitamin C is still unclear. Here, we investigated whether vitamin C alters epidermal ceramide content by regulating the expression and/or activity of its metabolic enzymes. When human keratinocytes were grown in 1.2 mM calcium with vitamin C (50 μg/ml) for 11 days, bulk ceramide content significantly increased in conjunction with terminal differentiation of keratinocytes as compared to vehicle controls (1.2 mM calcium alone). Synthesis of the ceramide fractions was enhanced by increased de novo ceramide synthesis pathway via serine palmitoyltransferase and ceramide synthase activations. Moreover, sphingosine-1-phosphate (S1P) hydrolysis pathway by action of S1P phosphatase was also stimulated by vitamin C supplementation, contributing, in part, to enhanced ceramide production. However, activity of sphingomyelinase, a hydrolase enzyme that converts sphingomyelin to ceramide, remained unaltered. Taken together, we demonstrate that vitamin C stimulates ceramide production in keratinocytes by modulating ceramide metabolic-related enzymes, and as a result, could improve overall epidermal barrier function. |
Databáze: | OpenAIRE |
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