The inhibitory effect of Gynostemma pentaphyllum on MCP-1 and type I procollagen expression in rat hepatic stellate cells
| Autor: | Qwa-Fun Wang, Ke-Yu Chen, Ming-Ho Chen, Shu-Hsin Chen, Jia-Jen Shee, Jung-Chou Chen, Yi-Wen Liu, Lih Geeng Chen, Jyan-Gwo J. Su |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Gene Expression Collagen Type I Rats Sprague-Dawley Drug Discovery Gene expression Hepatic Stellate Cells medicine Animals Gynostemma pentaphyllum Chemokine CCL2 Cell Proliferation Platelet-Derived Growth Factor Pharmacology biology Plant Extracts Cell growth Monocyte biology.organism_classification Molecular biology Gynostemma Rats Blot Procollagen peptidase medicine.anatomical_structure Biochemistry biology.protein Hepatic stellate cell Cytokines Platelet-derived growth factor receptor Drugs Chinese Herbal |
| Zdroj: | Journal of Ethnopharmacology. 126:42-49 |
| ISSN: | 0378-8741 |
| Popis: | Aim of the study Gynostemma pentaphyllum is a popular folk medicine that has been used for treatment of hepatitis in Asia. Our previous study demonstrates that Gynostemma pentaphyllum n-butanol extract inhibits the onset and improves the recovery of CCl4-induced liver fibrogenesis in rats and inhibits PDGF-induced rat hepatic stellate cells (HSCs) proliferation. In this study, the effect of Gynostemma pentaphyllum extract on cytokines and type I procollagen expression was analyzed. Materials and methods Rat HSCs were treated with PDGF, Gynostemma pentaphyllum n-butanol extract, RP-18-Gyp fraction, rapamycin or vehicle. Rat cytokine antibody array chip or ELISA kit was used for cytokines detection. Intracellular protein expression was detected by Western blotting, mRNA expression was analyzed by RT-PCR. Results RP-18-Gyp fraction is the more purified gypenosides fraction from Gynostemma pentaphyllum n-butanol extract. In cell proliferation, the inhibitory effect of 200 μg/ml RP-18-Gyp fraction is similar to 500 μg/ml Gynostemma pentaphyllum n-butanol extract. Furthermore, both of them have the ability of decreasing monocyte chemoattractant protein-1 (MCP-1) mRNA expression and protein release and inhibiting type I procollagen protein expression. Conclusions Both of Gynostemma pentaphyllum n-butanol extract and its more purified RP-18-Gyp fraction have the biological activities in the inhibition of cell proliferation, MCP-1 release and type I procollagen expression in rat HSCs. These data could provide the evidence to support for the traditional use of Gynostemma pentaphyllum in hepatitis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect