Design, Implementation, Simulation, and Visualization of a Highly Efficient RIM Microfluidic Mixer for Rapid Freeze-Quench of Biological Samples

Autor:	Taylor R. Page, Goher Mahmud, Thomas V. O'Halloran, Siowling Soh, Bryan Schmidt, Brian M. Hoffman, Bartosz A. Grzybowski, Sun Hee Kim
Rok vydání:	2011
Předmět:	Chaotic flow Chemistry business.industry Reaction injection molding Microfluidics Mixing (process engineering) Reynolds number Nanotechnology Atomic and Molecular Physics and Optics Article Visualization symbols.namesake symbols Process engineering business Mixing chamber Mathematical simulation
Zdroj:	Applied magnetic resonance. 40(4)
ISSN:	0937-9347
Popis:	Rapid freeze-quench (RFQ) trapping of short-lived reaction intermediates for spectroscopic study plays an important role in the characterization of biological reactions. Recently there has been considerable effort to achieve submillisecond reaction deadtimes. We present here a new, robust, high-velocity microfluidic mixer that enables such rapid freeze-quenching. It is a based on the mixing method of two impinging jets commonly used in reaction injection molding (RIM) of plastics. This method achieves efficient mixing by inducing chaotic flow at relatively low Reynolds numbers (Re =140). We present the first mathematical simulation and microscopic visualization of mixing in such RFQ micromixers, the results of which show that the impinging solutions efficiently mix within the mixing chamber. These tests, along with a practical demonstration in a RFQ setup that involves copper wheels, show this new mixer can in practice provide reaction deadtimes as low as 100 microseconds.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2fd72a62e6ad62a3883745c3a0260b3 https://pubmed.ncbi.nlm.nih.gov/22180701 Zobrazit plný text záznamu Full text from SpringerLink