Environmentally Sensitive Fluorescent Nucleoside Analogues for Surveying Dynamic Interconversions of Nucleic Acid Structures
Autor: | Lyes Zaghzi, Olga A. Zaporozhets, Viktoriia Y. Postupalenko, Yves Mély, Nicolas Barthes, Yitzhak Tor, Marianna Sholokh, Rajhans Sharma, Christian Boudier, Natalia Grytsyk, Alain Burger, Benoît Y. Michel, Dmytro Dziuba |
---|---|
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Protein Conformation 010402 general chemistry 7. Clean energy 01 natural sciences Catalysis Nucleobase 03 medical and health sciences chemistry.chemical_compound Protein structure Nucleic Acids Fluorescence Resonance Energy Transfer Nucleic acid structure Binding site Fluorescent Dyes Binding Sites Organic Chemistry Proteins Nucleosides General Chemistry 0104 chemical sciences Kinetics 030104 developmental biology Förster resonance energy transfer chemistry Biophysics Nucleic acid Nucleic Acid Conformation Thermodynamics Primer binding site DNA Protein Binding |
Zdroj: | Chemistry (Weinheim an der Bergstrasse, Germany). 24(52) |
ISSN: | 1521-3765 |
Popis: | Nucleic acids are characterized by a variety of dynamically interconverting structures that play a major role in transcriptional and translational regulation as well as recombination and repair. To monitor these interconversions, Forster resonance energy transfer (FRET)-based techniques can be used, but require two fluorophores that are typically large and can alter the DNA/RNA structure and protein binding. Additionally, events that do not alter the donor/acceptor distance and/or angular relationship are frequently left undetected. A more benign approach relies on fluorescent nucleobases that can substitute their native counterparts with minimal perturbation, such as the recently developed 2-thienyl-3-hydroxychromone (3HCnt) and thienoguanosine (th G). To demonstrate the potency of 3HCnt and th G in deciphering interconversion mechanisms, we used the conversion of the (-)DNA copy of the HIV-1 primer binding site (-)PBS stem-loop into (+)/(-)PBS duplex, as a model system. When incorporated into the (-)PBS loop, the two probes were found to be highly sensitive to the individual steps both in the absence and the presence of a nucleic acid chaperone, providing the first complete mechanistic description of this critical process in HIV-1 replication. The combination of the two distinct probes appears to be instrumental for characterizing structural transitions of nucleic acids under various stimuli. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |