| Autor: |
Lipeng Gao, Eve Qian Chen, Hong‐Bing Zhong, Jing Xie, Hong‐Zhi Song, Xu‐Bo Zhao, Jin‐Ran Lin, Qingmei Liu, Shihua Wang, Wen‐Yu Wu, Robert Chunhua Zhao, Xin‐Hua Liao |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Cytometry. Part A : the journal of the International Society for Analytical CytologyREFERENCES. 101(8) |
| ISSN: |
1552-4930 |
| Popis: |
Dermal papilla (DP) cells regulate hair follicle epithelial cells and melanocytes by secreting functional factors, playing a key role in hair follicle morphogenesis and hair growth. DP cells can reconstitute new hair follicles and induce hair regeneration, providing a potential therapeutic strategy for treating hair loss. However, current methods for isolating DP cells are either inefficient (physical microdissection) or only applied to genetically labeled mice. We systematically screened for the surface proteins specifically expressed in skin DP using mRNA expression databases. We identified two antibodies against receptors LEPTIN Receptor (LEPR ) and Scavenger Receptor Class A Member 5 (SCARA5) which could specifically label and isolate DP cells by flow cytometry from mice back skin at the growth phase. The sorted LEPR |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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