Increased Mucosal Production of Monomeric IgA1 but No IgA1 Protease Activity in Helicobacter pylori Gastritis
| Autor: | Audun Elnaes Berstad, K Valnes, Per Brandtzaeg, Mogens Kilian |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Immunoglobulin A Chronic gastritis Cell Count Helicobacter Infections Pathology and Forensic Medicine fluids and secretions stomatognathic system Leukocytes medicine Gastric mucosa Humans Aged Aged 80 and over Helicobacter pylori biology Serine Endopeptidases Middle Aged medicine.disease biology.organism_classification Urease Molecular biology Isotype J chain medicine.anatomical_structure Microscopy Fluorescence Gastric Mucosa Gastritis Immunoglobulin J-Chains Immunoglobulin A Secretory Immunology biology.protein Female Antibody Immunoglobulin J Chain Regular Articles |
| Zdroj: | Berstad, A E, Kilian, M, Valnes, K N & Brandtzaeg, P 1999, ' Increased mucosal production of monomeric IgA1 but no IgA1 protease activity in Helicobacter pylori gastritis ', American Journal of Pathology, vol. 155, no. 4, pp. 1097-104 . |
| ISSN: | 0002-9440 |
| DOI: | 10.1016/s0002-9440(10)65212-1 |
| Popis: | Udgivelsesdato: 1999-Oct Immunoglobulin A and IgM are subjected to epithelial transport only when they are produced as polymers with incorporated J chain. Immunocytes containing various Ig isotypes and associated J chain in gastric mucosa, as well as IgA-degrading protease activity in Helicobacter pylori cultures, were examined. Gastric body specimens from 15 H. pylori-positive and 14 H. pylori-negative patients were studied by paired immunofluorescence for IgA, IgA1, IgA2, IgG, or IgM and concurrent cellular J chain. H. pylori isolates were incubated with IgA1 or secretory IgA and examined by immunoelectrophoresis for cleavage products. A substantial increase of Ig-producing cells occurred in chronic gastritis, particularly in the IgA1 isotype, but H. pylori was shown to possess neither IgA1-specific nor nonspecific IgA-degrading protease activity. Regardless of infection status, reduced J chain expression was observed for all immunocyte isotypes (except for IgM) in inflamed compared with normal gastric body mucosa, the median positivity for IgA1 cells being reduced to 58.7% versus 87.9% (P = 0.0002), and for IgA2 cells to 48.9% versus 87.8% (P = 0.0002). This down-regulation of the J chain suggested that a large fraction of IgA monomers is produced in gastritis. |
| Databáze: | OpenAIRE |
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