Folylpolyglutamate synthetase from beef liver: purification and some properties

Autor:	Z. B. Pristupa, C. M. McAuley, K. G. Scrimgeour
Rok vydání:	1991
Předmět:	chemistry.chemical_classification Antiserum Blotting Western Cell Biology Polypeptide chain Biology Biochemistry Molecular biology Enzyme Liver chemistry Beef Liver FOLYLPOLYGLUTAMATE SYNTHETASE Animals Cattle Electrophoresis Polyacrylamide Gel Amino Acids Peptide Synthases Molecular Biology
Zdroj:	Biochemistry and Cell Biology. 69:556-560
ISSN:	1208-6002 0829-8211
DOI:	10.1139/o91-082
Popis:	The polypeptide chain of folylpolyglutamate synthetase from beef liver has been isolated and partially characterized. This polypeptide has an apparent molecular weight of 73 000. Its amino-terminal residue is blocked. Amino acid analysis agrees with the hydrophobic properties and the pi (6.0) of this cytosolic enzyme. Polyclonal antibodies to the denatured enzyme have been prepared.Key words: folylpolyglutamate synthetase, folates, polyglutamates, electrophoresis, antisera.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2e531d4b5472ea09aefd0bb4039ce5d https://doi.org/10.1139/o91-082 Zobrazit plný text záznamu