The role of STIM1/ORAI1 channel in the analgesic effect of grain-sized moxibustion on inflammatory pain mice model
| Autor: | Si-rui Lin, Ruizhen Huang, Chuan-Yi Zuo, Han-Xiao Zhang, Cheng-Shun Zhang, Xiao-qin Dai, Peng Lv, Gang Shi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
inorganic chemicals
0301 basic medicine Male ORAI1 Protein Moxibustion medicine.medical_treatment Stimulation Pharmacology Immunofluorescence 030226 pharmacology & pharmacy General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Mice 0302 clinical medicine Western blot Medicine Animals Pain Management Stromal Interaction Molecule 1 General Pharmacology Toxicology and Pharmaceutics Inflammation medicine.diagnostic_test business.industry ORAI1 Therapeutic effect STIM1 General Medicine Inflammatory pain Mice Inbred C57BL Disease Models Animal 030104 developmental biology business |
| Zdroj: | Life sciences. 280 |
| ISSN: | 1879-0631 |
| Popis: | The therapeutic effect of grain-sized moxibustion (GS-Moxi) on inflammatory pain has been well recognized clinically, but the mechanism remains unclear. STIM1/ORAI1 is a sensible temperature channel, therefore; this study aimed to investigate the analgesic effect of GS-Moxi and the association with STIM1/ORAI1 expression. CFA-induced inflammatory pain model was established and was treated with GS-Moxi after 3 days of CFA injection. The behavioral test was measured after the GS-Moxi; then, serum was prepared for IL-1β, IL-6, and TNF-α, and the stimulated skin was used for measuring STIM1 and ORAI1 expression. The results indicated GS-Moxi had an analgesic effect on inflammatory pain and the heat variation was significant for the analgesia. GS-Moxi decreased the expression of IL-1β, IL-6, and TNF-α. Immunofluorescence and western blot analysis illustrated that heat change was associated with the stimulation of STIM1 and ORAI1. Suggesting that heat variation created by GS-Moxi could be crucial in this therapy and STIM1 and ORAI1 were potential enhancers in regulating analgesia of GS-Moxi. |
| Databáze: | OpenAIRE |
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