Differential Proteomic Analysis of Syncytiotrophoblast Extracellular Vesicles from Early-Onset Severe Preeclampsia, using 8-Plex iTRAQ Labeling Coupled with 2D Nano LC-MS/MS

Autor: Yinfeng Li, Jianxin Guo, Jing Yang, Hongmei Li, Yingru Zheng, Zhiling Yang, Lei Han, Lijuan Zhou, Li Li, Xiaojie Liu, Yan Gu, Meijia Yu, Li Yilin, Wei Huang, Hu Jiongyu, Xin Zhang, Jian Han
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Adult
Proteomics
Time Factors
Proteome
Physiology
Citric Acid Cycle
Cell Culture Techniques
Mitochondrion
Biology
Microparticles
Exosomes
Severity of Illness Index
lcsh:Physiology
lcsh:Biochemistry
Extracellular Vesicles
Syncytiotrophoblast
Western blot
Pre-Eclampsia
Pregnancy
Tandem Mass Spectrometry
Syncytiotrophoblast extracellular vesicles
medicine
Humans
lcsh:QD415-436
medicine.diagnostic_test
Staining and Labeling
lcsh:QP1-981
Gene Expression Profiling
Fatty Acids
Gluconeogenesis
Membrane Transport Proteins
Molecular Sequence Annotation
Membrane transport
Preeclampsia
Molecular biology
Cell biology
Trophoblasts
Gene expression profiling
medicine.anatomical_structure
Gene Expression Regulation
iTRAQ
Fatty acid elongation
Transmembrane transporter activity
Female
Glycolysis
Microvesicles
Zdroj: Cellular Physiology and Biochemistry, Vol 36, Iss 3, Pp 1116-1130 (2015)
ISSN: 1421-9778
1015-8987
Popis: Aims: Previous studies have revealed that the increased shedding of syncytiotrophoblast extracellular vesicles (STBM) may lead to preeclampsia (PE). We aimed to identify the proteins carried by STBM and their potential pathological roles in early-onset severe PE. Methods: In this study, we performed a differential proteomic analysis of STBM from early-onset severe PE patients, using iTRAQ isobaric tags and 2D nano LC-MS/MS. STBM were generated by the in vitro explant culture method, and then verified by electron microscopy and western blot analysis. Results: A total of 18 533 unique peptides and 3 317 proteins were identified, 3 292 proteins were quantified. We identified 194 differentially expressed proteins in STBM from early-onset severe PE patients, 122 proteins were up-regulated and 72 proteins were down-regulated. Further bioinformatics analysis revealed that mitochondrion, transmembrane transport and transmembrane transporter activity were the most abundant categories in gene ontology (GO) annotation. Glycolysis/ gluconeogenesis, citrate cycle, fatty acid elongation, steroid hormone biosynthesis and oxidative phosphorylation were the five significantly represented pathways. Four differentially expressed proteins (siglec-6, calnexin, CD63 and S100-A8) related to inflammation, coagulation or immunoregulation were independently verified using western blot. Conclusions: The identification of key proteins carried by STBM may serve not only as a basis for better understanding and further exploring the etiology and pathogenesis of PE, but also as potential biomarkers and in providing targets for future therapy in PE, especially in early-onset severe PE(sPE).
Databáze: OpenAIRE
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