Intercalation of curcumin into liposomal chemotherapeutic agent augments apoptosis in breast cancer cells
| Autor: | Fatemeh Hassandokht, Abazar Roustazadeh, Ali Mousavizadeh, Mohsen Saghebray Shirazi, Hassan Bardania, Mehzad Jafari Barmak, Mohsen Alipour, Mohsen Nikseresht, Shabnam Tarvirdipour, Reza Mahmoudi, Maryam Tajali Ardakani, Bahman Karimi, Marzieh Baneshi |
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| Rok vydání: | 2020 |
| Předmět: |
Curcumin
Ideal system Cell Survival Intercalation (chemistry) Biomedical Engineering Antineoplastic Agents Apoptosis Breast Neoplasms 02 engineering and technology 030226 pharmacology & pharmacy Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Breast cancer medicine Humans Cytotoxicity Drug Carriers Liposome Chemistry Drug Synergism 021001 nanoscience & nanotechnology medicine.disease Drug Liberation Liposomes MCF-7 Cells Cancer research Nanoparticles Female Breast cancer cells Cisplatin 0210 nano-technology |
| Zdroj: | Journal of Biomaterials Applications. 35:1005-1018 |
| ISSN: | 1530-8022 0885-3282 |
| Popis: | Resistance to common chemotherapeutic agents is a frequent phenomenon in late-stage breast cancers. An ideal system capable of the co-delivery of hydrophobic and hydrophilic chemotherapeutic agents can regulate the dosage and co-localization of pharmaceutical compounds and thereby improve the anticancer efficacy. Here, for the first time, we have intercalated curcumin (Cur) into a double-layered membrane of cisplatin (Cis) liposomes to obtain a dosage controlled co-delivery formulation, capable of inducing apoptosis in breast cancer cells. The concentrations of Cur and Cis in nanoliposome (Cur-Cis@NLP) were optimized by response surface methodology (RSM); RSM optimization showed 99.81 and 23.86% entrapment efficiency for Cur and Cis, respectively. TEM analysis demonstrated the fabrication of nanoparticles with average diameter of 100 nm. The anticancer and apoptotic effects of Cur-Cis@NLPs were also evaluated using MTT assay, fluorescent staining and flow cytometry assays. Cytotoxicity assessments of various Cur-Cis@NLPs concentrations demonstrated a concentration-dependent manner. In comparison to free and liposomal Cis, Cur-Cis@NLP reduced breast cancer cells’ viability (82.5%) in a significant manner at a final concentration of 32 μg.mL−1 and 20 μg.mL−1 of Cur and Cis, respectively. Combination index values calculation of Cur-Cis@NLP showed an overall CI value |
| Databáze: | OpenAIRE |
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