Anti-inflammatory activity, cytotoxicity and active compounds ofTinospora smilacina Benth
| Autor: | Rachel W. Li, G. David Lin, David N Leach, Gregory J. Leach, Peter G. Waterman, Stephen P Myers, Donald J. Brushett |
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| Rok vydání: | 2004 |
| Předmět: |
Tinospora
medicine.drug_class Lipoxygenase HL-60 Cells Plant Roots Phospholipases A Anti-inflammatory law.invention Inhibitory Concentration 50 Phospholipase A2 law Cell Line Tumor Animals Humans Bioassay Medicine Cytotoxicity Pharmacology chemistry.chemical_classification Sheep Plant Stems biology Traditional medicine Plant Extracts business.industry Anti-Inflammatory Agents Non-Steroidal Membrane Proteins Fatty acid Antineoplastic Agents Phytogenic Isoenzymes Hep G2 Biochemistry chemistry Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases Arachidonate 5-lipoxygenase Cyclooxygenase 1 biology.protein Cattle business Phytotherapy |
| Zdroj: | Phytotherapy Research. 18:78-83 |
| ISSN: | 1099-1573 0951-418X |
| DOI: | 10.1002/ptr.1373 |
| Popis: | Tinospora smilacina Benth. has been used in Australian indigenous medicine for the treatment of headache, rheumatoid arthritis and other inflammatory disorders. As part of an investigation into the anti-inflammatory potential of plants using an ethnopharmacological approach, the present study sought to evaluate the efficacy and safety of Tinospora smilacina. An ethanol extract of this plant was evaluated in vitro for anti-inflammatory activities on cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LO) and phospholipase A(2) (PA(2)). The ethanol extract of Tinospora smilacina showed inhibitory activities on COX-1, COX-2, 5-LO and PA(2) with the IC(50) values of 63.5, 81.2, 92.1 and 30.5 micro g/mL respectively. Cytotoxic effect of the extracts of Tinospora smilacina was investigated in vitro using ATP-based luminescence assay and the results showed no cytotoxic effect on cell lines of skin fibroblasts (1BR3), human Caucasian hepatocyte carcinoma (Hep G2) and human Caucasian promyelocytic leukaemia (HL-60). This paper also describes the results of fractionations and bioassay guided chemical studies, suggesting that the anti-inflammatory activity is due to triterpene-fatty acid esters and free fatty acids. |
| Databáze: | OpenAIRE |
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