Reporter proteins for in vivo fluorescence without oxygen
| Autor: | Franco Circolone, Marion Wendorff, Ulrich Krauss, Thorsten Eggert, Thomas Drepper, Achim Heck, Wolfgang Gärtner, Jan-Karl Guterl, Karl-Erich Jaeger, Aba Losi |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Flavin Mononucleotide
Biomedical Engineering Flavin mononucleotide Bioengineering Bacillus subtilis Applied Microbiology and Biotechnology Fluorescence Rhodobacter capsulatus Green fluorescent protein Bimolecular fluorescence complementation chemistry.chemical_compound Bacterial Proteins In vivo Genes Reporter Anaerobiosis Luminescent Proteins biology Pseudomonas putida biology.organism_classification Protein Structure Tertiary Oxygen Biochemistry chemistry Molecular Medicine Biotechnology |
| Zdroj: | Nature biotechnology. 25(4) |
| ISSN: | 1087-0156 |
| Popis: | Fluorescent reporter proteins such as green fluorescent protein are valuable noninvasive molecular tools for in vivo real-time imaging of living specimens. However, their use is generally restricted to aerobic systems, as the formation of their chromophores strictly requires oxygen. Starting with blue-light photoreceptors from Bacillus subtilis and Pseudomonas putida that contain light-oxygen-voltage-sensing domains, we engineered flavin mononucleotide-based fluorescent proteins that can be used as fluorescent reporters in both aerobic and anaerobic biological systems. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|