Maintenance of Normal T Lymphocyte Function after Transfection with SV40 Large T
| Autor: | Q C Ryan, Donna M. Murasko, I M Goonewardene |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
animal structures
Cell Survival Antigens Polyomavirus Transforming T-Lymphocytes viruses T cell Immunology Gene Expression Cell Separation In Vitro Techniques Biology Lymphocyte Activation Transfection Interferon-gamma Interleukin 21 Antigen T-Lymphocyte Subsets Receptors Transferrin medicine Humans Cytotoxic T cell RNA Messenger In Situ Hybridization fungi CD28 Receptors Interleukin-2 T lymphocyte Cell Transformation Viral Molecular biology medicine.anatomical_structure Interleukin-2 Leukocyte Common Antigens CD8 |
| Zdroj: | Cellular Immunology. 149:65-81 |
| ISSN: | 0008-8749 |
| DOI: | 10.1006/cimm.1993.1136 |
| Popis: | Human T lymphocytes in culture undergo cellular senescence similar to that observed in fibroblasts. We recently demonstrated that senescence of T lymphocytes can be postponed by transfection with SV40 large T. In the current study, we examine the functional activity of SV40 large T transfected T lymphocytes. Transfected T cells of both young and elderly subjects appear to display normal T cell function: they cease doubling upon removal of IL-2; in the presence of autologous adherent mononuclear cells they respond to mitogen stimulation and produce IL-2 and IFN-gamma during proliferation; and they express both IL-2 and transferrin receptors similar to those observed in mitogen-stimulated nontransfected T cells. In addition, more than 90% of the transfected cells express both the CD4 and "naive" T cell marker, CD45RA. In order to investigate whether the lack of long-lived CD8+ cells reflects phenotypic restriction, separated CD4+ and CD8+ subpopulations were transfected with SV40 large T. All transfections resulted in extended life spans of CD4+, but not CD8+, cells. Therefore, although SV40 large T transfected T lymphocytes maintain normal function, while demonstrating an extended life span, the effect may be restricted to CD4+ cells. |
| Databáze: | OpenAIRE |
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