MicroRNA-939 down-regulates CD2-associated protein by targeting promoter in HEK-293T cells
| Autor: | Yu Ping Huang, Ling Zhi Qiu, Guo Ping Zhou |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
CD2-Associated Protein Down-Regulation Biology Critical Care and Intensive Care Medicine 03 medical and health sciences microRNA Gene expression Humans Promoter Regions Genetic Cells Cultured Adaptor Proteins Signal Transducing Regulation of gene expression Messenger RNA Reporter gene Podocytes Promoter General Medicine Molecular biology Cytoskeletal Proteins MicroRNAs 030104 developmental biology HEK293 Cells Gene Expression Regulation Nephrology Slit diaphragm |
| Zdroj: | Renal failure. 38(4) |
| ISSN: | 1525-6049 |
| Popis: | CD2-associated protein (CD2AP) serves as a slit diaphragm (SD) protein and plays essential roles in maintaining podocyte integrity and reducing proteinuria. MicroRNAs (miRNAs) are novel regulators of gene expression. Podocyte-specific loss of miRNAs would lead to significant proteinuria. Here, we report new evidence in which miRNAs may function to suppress CD2AP expression through a transcriptional way. By scanning human CD2AP promoter in silico for sequences complementary to known miRNAs, we chose miR-939, miR-148b*, miR-191*, miR-638 as four candidates and transfected them into HEK-293T cells. Dual-luciferase reporter assay identified that only miR-939 significantly reduced the relative luciferase activity of CD2AP promoter region. Further analysis confirmed that the mRNA and protein expressions of CD2AP were also down-regulated by miR-939. In conclusion, we have identified that miR-939 targets CD2AP promoter sequences and suppresses its gene expression. These findings suggest that miRNAs may mediate podocyte injury via reducing the expression of the SD proteins, such as CD2AP. |
| Databáze: | OpenAIRE |
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