Trypanosoma cruzi Modulates PIWI-Interacting RNA Expression in Primary Human Cardiac Myocytes during the Early Phase of Infection
| Autor: | Kayla J Rayford, Ashutosh Arun, Yulia Kleschenko, Maria F. Lima, Ayorinde Cooley, Siddharth Pratap, Pius N. Nde, Girish Rachakonda, Fernando Villalta |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
endocrine system Chagas disease NFATC2 In silico Trypanosoma cruzi Piwi-interacting RNA Catalysis Article Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences 0302 clinical medicine Gene expression parasitic diseases human cardiac myocytes Physical and Theoretical Chemistry Molecular Biology Gene lcsh:QH301-705.5 Spectroscopy Regulation of gene expression biology urogenital system Organic Chemistry fibrosis parasite pathogenesis RNA piRNome General Medicine biology.organism_classification Computer Science Applications Cell biology 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 piRNAs 030220 oncology & carcinogenesis |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 9439, p 9439 (2020) International Journal of Molecular Sciences Volume 21 Issue 24 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Trypanosoma cruzi dysregulates the gene expression profile of primary human cardiomyocytes (PHCM) during the early phase of infection through a mechanism which remains to be elucidated. The role that small non-coding RNAs (sncRNA) including PIWI-interacting RNA (piRNA) play in regulating gene expression during the early phase of infection is unknown. To understand how T. cruzi dysregulate gene expression in the heart, we challenged PHCM with T. cruzi trypomastigotes and analyzed sncRNA, especially piRNA, by RNA-sequencing. The parasite induced significant differential expression of host piRNAs, which can target and regulate the genes which are important during the early infection phase. An average of 21,595,866 (88.40%) of clean reads mapped to the human reference genome. The parasite induced 217 unique piRNAs that were significantly differentially expressed (q &ge 0.8). Of these differentially expressed piRNAs, 6 were known and 211 were novel piRNAs. In silico analysis showed that some of the dysregulated known and novel piRNAs could target and potentially regulate the expression of genes including NFATC2, FOS and TGF-&beta 1, reported to play important roles during T. cruzi infection. Further evaluation of the specific functions of the piRNAs in the regulation of gene expression during the early phase of infection will enhance our understanding of the molecular mechanism of T. cruzi pathogenesis. Our novel findings constitute the first report that T. cruzi can induce differential expression of piRNAs in PHCM, advancing our knowledge about the involvement of piRNAs in an infectious disease model, which can be exploited for biomarker and therapeutic development. |
| Databáze: | OpenAIRE |
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