Metabolic signature profiling as a diagnostic and prognostic tool in paediatric Plasmodium falciparum malaria
| Autor: | Maria Nelson, Patrick Kyamanwa, Sven Bergström, Izabella Surowiec, Ben Karenzi, Judy Orikiiriza, Johan Normark, Elisabeth Karlsson, Mari Bonde, Johan Trygg |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Infectious Medicine Metabolite malaria Infektionsmedicin Disease macromolecular substances Bioinformatics Major Articles chemistry.chemical_compound Metabolomics Internal medicine parasitic diseases medicine Plasmodium berghei Bioinformatics (Computational Biology) biology business.industry Plasmodium falciparum medicine.disease biology.organism_classification metabolomics disease staging Infectious Diseases Oncology chemistry Cohort Bioinformatik (beräkningsbiologi) business Malaria Disease staging |
| Zdroj: | Open Forum Infectious Diseases |
| Popis: | The metabolic profile in paediatric patients suffering from acute P. falciparum malaria carries sufficient information to grade disease severity. Background. Accuracy in malaria diagnosis and staging is vital to reduce mortality and post infectious sequelae. In this study, we present a metabolomics approach to diagnostic staging of malaria infection, specifically Plasmodium falciparum infection in children. Methods. A group of 421 patients between 6 months and 6 years of age with mild and severe states of malaria with age-matched controls were included in the study, 107, 192, and 122, individuals, respectively. A multivariate design was used as basis for representative selection of 20 patients in each category. Patient plasma was subjected to gas chromatography-mass spectrometry analysis, and a full metabolite profile was produced from each patient. In addition, a proof-of-concept model was tested in a Plasmodium berghei in vivo model where metabolic profiles were discernible over time of infection. Results. A 2-component principal component analysis revealed that the patients could be separated into disease categories according to metabolite profiles, independently of any clinical information. Furthermore, 2 subgroups could be identified in the mild malaria cohort who we believe represent patients with divergent prognoses. Conclusions. Metabolite signature profiling could be used both for decision support in disease staging and prognostication. |
| Databáze: | OpenAIRE |
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