Detection of Donor-specific DNA Polymorphisms in the Urine of Renal Transplant Recipients
| Autor: | Sinuhe Hahn, Thomson Pd, Xiao Yan Zhong, Wolfgang Holzgreve, Ying Li, Deirdré Hahn |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Transplantation Chimera
Kidney Polymorphism Genetic Urinary system Biochemistry (medical) Clinical Biochemistry DNA Urine Biology Y chromosome Kidney Transplantation Polymerase Chain Reaction Molecular biology Tissue Donors DNA sequencing Transplantation Andrology medicine.anatomical_structure Tandem Repeat Sequences Genetic marker medicine Humans Microsatellite |
| Zdroj: | Clinical Chemistry. 49:655-658 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | Recently, a novel form of chimerism, termed urinary DNA chimerism, has been described in kidney transplant recipients in that cell-free DNA from the donor kidney was detected in the recipient’s urine (1). Quantitative analysis of this urinary donor-derived DNA has indicated that it may serve as a new marker to monitor kidney transplant engraftment because increased concentrations were present under conditions of graft rejection, which decreased to basal values after immunosuppressive treatment (2). A caveat of these studies was that they relied on sex-disparate donor–recipient conditions: because the PCR assays used were specific for the Y chromosome, cell-free DNA from the donor kidney could be detected only in the urine of female recipients who had received male kidneys (1)(2). We examined whether other kidney donor-derived DNA sequences could be detected in the urine of transplant recipients, using PCR assays specific for highly polymorphic short tandem repeat (STR) loci, also termed microsatellite markers. Previous examinations using such polymorphic genetic loci have shown that they can be used for differentiating female fetal cells from maternal ones (3)(4) or for the gender-independent detection of cell-free fetal DNA in maternal plasma (5)(6). For this purpose, we tested for the presence of donor-specific STR loci in the urine of cases in which the donor and recipient were either of the same sex or the donor was female and the recipient was male. For our study, which was approved by our respective ethics review boards, five cases involving living-donor (four related and one unrelated) transplants were enrolled. Blood samples … |
| Databáze: | OpenAIRE |
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