courtless, the Drosophila UBC7 Homolog, Is Involved in Male Courtship Behavior and Spermatogenesis
| Autor: | Daniel L. Segal, Ralph J. Greenspan, Galit Rosenfeld, Sara Orgad |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Male
Molecular Sequence Data Mutant Gene Expression Biology Transfection medicine.disease_cause Animals Genetically Modified Ligases P element Sexual Behavior Animal Genetics Homologous chromosome medicine Animals Drosophila Proteins Cloning Molecular Peptide Synthases Spermatogenesis Ubiquitins Gene Alleles Polytene chromosome band Mutation Base Sequence Sequence Homology Amino Acid Gene map Homozygote Courtship Physical Chromosome Mapping Meiosis Ubiquitin-Conjugating Enzymes Insect Proteins Drosophila Female Drosophila Protein Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1943-2631 |
| DOI: | 10.1093/genetics/155.3.1267 |
| Popis: | The courtless (col) mutation disrupts early steps of courtship behavior in Drosophila males, as well as the development of their sperm. Most of the homozygous col/col males (78%) do not court at all. Only 5% perform the entire ritual and copulate, yet these matings produce no progeny. The col gene maps to polytene chromosome band 47D. It encodes two proteins that differ in their carboxy termini and are the Drosophila homologs of the yeast ubiquitin-conjugating enzyme UBC7. The col mutation is caused by an insertion of a P element into the 3′ UTR of the gene, which probably disrupts translational regulatory elements. As a consequence, the homozygous mutants exhibit a six- to sevenfold increase in the level of the COL protein. The col product is essential, and deletions that remove the col gene are lethal. During embryonic development col is expressed primarily in the CNS. Our results implicate the ubiquitin-mediated system in the development and function of the nervous system and in meiosis during spermatogenesis. |
| Databáze: | OpenAIRE |
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